不同稀释介质对化学发光免疫法测定肺炎支原体抗体的影响  

The impact of different dilution media on the determination of Mycoplasma pneumoniae antibody(IgG)by chemiluminescence immunoassay

作　　者：张建平[1] 姚辉 胡文杰 ZHANG Jianping;YAO Hui;HU Wenjie(Clinical Laboratory,Affiliated Maternity and Child Health Care Hospital of Nantong University,Nantong,jiangsu 226004,China)

机构地区：[1]南通大学附属妇幼保健院检验科,江苏南通226004

出　　处：《国际检验医学杂志》2023年第S02期124-127,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨采用不同稀释介质对化学发光免疫法稀释测定肺炎支原体(MP)抗体(IgG)结果的影响。方法使用全自动化学发光测定仪测定60例血清标本,其中阴性(<24 AU/mL)、可疑(24~36 AU/mL)、阳性(>36 AU/mL)结果各20例。分别使用配套稀释液、生理盐水、蒸馏水作为稀释介质,进行1∶19(标本30 uL+稀释液570 uL)的手工稀释,再上机检测,结果进行统计学分析。结果各组数据经Kolmogorov-Smirnov正态性分析,原始值,配套稀释液、生理盐水、蒸馏水稀释测定值均不具有正态分布特性(均P<0.05);各稀释介质(配套稀释液、生理盐水、蒸馏水)稀释测定结果与原始结果经Spearman相关性分析相关系数,并使用线性回归模型校正稀释测定结果;Wilcoxon配对检验显示,配套稀释液组校正前与校正后的结果、生理盐水组和蒸馏水组校正后的结果与原始结果的差异无统计学意义(P均>0.05);经Bland Altman一致性检验分析,以原始结果阴性组、可疑组、阳性组的±(均值×1/3允许总误差)作为结果一致性界限的评价标准,配套稀释液组校正前、校正后的结果与原始结果差值符合评价标准的百分比分别为:阴性组100%、100%;可疑组100%、100%;阳性组95%、95%。生理盐水组和蒸馏水组校正后的结果与原始结果差值小于评价标准的百分比分别为:阴性组0%、0%;可疑组55%、10%;阳性组70%、45%。配套稀释液稀释测定的结果在校正前和校正后结果与原始结果一致性符合要求。结论在三种稀释介质稀释测定MP-IgG的结果中,配套稀释液稀释测定的结果满足较少标本即可检测的要求,且无需校正,与原始结果一致性好,可作为稀释测定MP-IgG的最优稀释液。Objective To explore the influence of different dilution media on the results of dilution and determination of antibody IgG against Mycoplasma pneumonia by chemiluminescence immunoassay.Methods Determination of 60 serum samples with automatic chemiluminescence analyzer(The results were negative(<24 AU/mL),suspicious(24 au/mL-36 AU/mL)and positive(>36 AU/mL)in 20 cases each),Use matching diluent,physiological saline and distilled water as dilution medium respectively,Conduct 1:19(sample 30μL+diluent 570μL)manual dilution,and then test on the machine,Results were statistically analyzed.Results According to Kolmogorov-Smirnov normality analysis,the original value,the measured value of matched diluent,physiological saline and distilled water dilution did not have normal distribution characteristics(P<0.05).Through Spearman correlation analysis,the correlation coefficients Rs of the dilution test results of each dilution medium(matching diluent,physiological saline,distilled water)and the original results and the dilution test results were corrected by linear regression model.Wilcoxon paired test showed that there was no statistically significant difference between the corrected and original results of the matched diluent group,the normal saline group and the distilled water group(P all>0.05).According to the analysis of Bland Altman consistency test,±(mean×1/3 total allowable error)as the evaluation standard for the consistency limit of the results,the percentage of the difference between the results before and after correction and the original results of the matched diluent group meeting the evaluation standard is 100%and 100%respectively;100%and 100%of suspicious group;The positive group was 95%and 95%.The percentage of the difference between the corrected results and the original results of the normal saline group and the distilled water group is less than the evaluation standard:0%in the negative group,0%in the distilled water group;55%and 10%of the suspicious group;Positive group 70%,45%.The results of the d

关 键 词：肺炎支原体 抗体 稀释介质 化学发光免疫法 

分 类 号：R563.1[医药卫生—呼吸系统] R446.6[医药卫生—内科学]