基于生物信息学分析ALDH2对肺腺癌的预后价值评估  

Prognostic value assessment of ALDH2 in lung adenocarcinoma based on bioinformatics

作　　者：申效榕 闫梓翼 黄园莉 朱清[1] 张光辉 慈红非 吴琼[1] 高琴[2] 吴礼高[1] Shen Xiaorong;Yan Ziyi;Huang Yuanli;Zhu Qing;Zhang Guanghui;Ci Hongfei;Wu Qiong;Gao Qin;Wu Ligao(Department of Pathology,Bengbu Medical College&Department of Pathology,the First Affiliated Hospital of Bengbu Medical College;Department of physiology,Bengbu Medical College,Bengbu,Anhui,233000,China)

机构地区：[1]蚌埠医学院病理学教研室,蚌埠医学院第一附属医院病理科,安徽蚌埠233000 [2]蚌埠医学院生理学教研室

出　　处：《齐齐哈尔医学院学报》2023年第19期1801-1808,共8页Journal of Qiqihar Medical University

基　　金：安徽高校自然科学研究重点项目(KJ2021A078)。

摘　　要：目的分析乙醛脱氢酶基因(ALDH2)在肺腺癌(LUAD)中的表达对其预后的影响。方法通过生物信息学,利用从TCGA数据库下载的肺腺癌RNA及临床资料,分析ALDH2基因在多种癌症中的表达情况,以及其在配对和非配对的肺腺癌样本中的表达,用免疫组化的方法验证ALDH2在肺腺癌中的表达。采用Kaplan-Meier方法和Cox回归分析ALDH2在LUAD中的临床特征关系及预后价值。用免疫棒棒糖图显示ALDH2与免疫浸润关系。结果对于TCGA数据库下载的数据进行分析,可得到ALDH2在33种癌症中的表达情况,发现ALDH2在多形成性胶质细胞瘤、子宫内膜癌、乳腺浸润癌、肺腺癌、食管癌、胃和食管癌、混合肾癌、前列腺癌、胃癌、头颈鳞状细胞癌、肾透明细胞癌、肺鳞癌、肝细胞癌、肾母细胞瘤、黑色素瘤、膀胱尿路上皮癌、甲状腺癌、卵巢浆液性囊腺癌、子宫肉瘤、白血病、急性髓细胞样白血病、嗜铬细胞瘤和副神经节瘤、肾上腺皮质癌、肾嫌色细胞癌和胆管癌中低表达(P<0.001);在胶质瘤、脑低级别胶质瘤、肾乳头状细胞癌、结肠癌、结直肠癌和胰腺癌中表达上升(P<0.001);对肺腺癌及正常组织进行分析,发现ALDH2在LUAD中低表达(P<0.001);在肺腺癌中ALDH2的表达与患者的ALDH2的表达水平与肿瘤T分期(P=0.041)、淋巴结转移(P=0.009)、病理分级(P=0.037)、年龄(P=0.038)以及吸烟史(P=0.019)等临床特征显著相关;将肺腺癌患者分为高、低表达ALDH2两组,总体生存时间(OS)、疾病特异性生存期(DSS)、无进展间隔(PFI)、首次进展生存期(FP)、无复发生存期(RFS)具有显著差异;ROC分析ALDH2基因表达水平对预测患者有较好敏感性;ALDH2表达与免疫浸润的CD8+T细胞、T细胞、辅助性T细胞、CD56bright NK细胞、肥大细胞、嗜酸粒细胞和DC细胞显著相关;免疫组化显示LUAD中存在ADH2低表达。结论ALDH2在肺腺癌中低表达,与其临床特征和患者预后�Objective To analyze the expression of aldehyde dehydrogenase 2(ALDH2)gene in lung adenocarcinoma(LUAD)and its impact on the prognosis.Methods Through bioinformatics analysis,the expression of ALDH2 gene in various cancers and paired and none-paired samples were analyzed by utilizing lung adenocarcinoma RNA and clinical data downloaded from the TCGA database.We verified the expression of ALDH2 in lung adenocarcinoma using immunohistochemistry.Kaplan-Meier method and Cox regression analysis were used to analyze the clinical characteristics and prognostic value of ALDH2 in LUAD.The relationship between ALDH2 and immune infiltration was displayed using an immune plot.Results Analysis of the data downloaded from the TCGA database reveals the expression of ALDH2 in 33 types of cancer.It was found that ALDH2 was down-regulated in glioblastoma multiforme,endometrial cancer,invasive breast cancer,lung adenocarcinoma,esophageal cancer,gastric and esophageal cancer,mixed renal cell carcinoma,prostate cancer,gastric cancer,head and neck squamous cell carcinoma,clear cell renal cell carcinoma,lung squamous cell carcinoma,hepatocellular carcinoma,Wilms tumor,melanoma,bladder urothelial carcinoma,thyroid cancer,ovarian mucinous cystadenocarcinoma,uterine sarcoma,leukemia,acute myeloid leukemia,pheochromocytoma and paraganglioma,adrenocortical carcinoma,renal chromophobe cell carcinoma,and cholangiocarcinoma(P<0.001).On the other hand,ALDH2 expression was up-regulated in gliomas,low-grade gliomas,papillary renal cell carcinoma,colon cancer,rectal cancer,and pancreatic cancer(P<0.001).In the analysis of lung adenocarcinoma and normal tissues,ALDH2 was found to be under-expressed in LUAD(P<0.001).Furthermore,ALDH2 expression in lung adenocarcinoma was significantly associated with clinical characteristics such as tumor T stage(P=0.041),lymph node metastasis(P=0.009),pathological grade(P=0.037),age(P=0.038),and smoking history(P=0.019).Patients with lung adenocarcinoma were divided into high and low ALDH2 expression groups,and sig

关 键 词：乙醛脱氢酶2基因(ALDH2) 肺腺癌 TCGA数据库 生存预后 

分 类 号：R734.2[医药卫生—肿瘤]