甘蓝广谱抗根肿病材料的鉴定和创制  

Identification and Creation of Broad-spectrum Clubroot Resistance Materials

作　　者：卢淑锦 朱明钊 张娜 方智远[2] 杨丽梅[2] 庄木[2] 张扬勇[2] 吕红豪[2] 季家磊 闫继平 王勇 LU Shujin;ZHU Mingzhao;ZHANG Na;FANG Zhiyuan;YANG Limei;ZHUANG Mu;ZHANG Yangyong;LUY Honghao;JI Jialei;YAN Jiping;WANG Yong*(〔1College of Horticulture,Hunan Agricultural University,Ministry of Education Engineering Research Center of Germplasm Innovation and New Variety Breeding of Horticultural Crops,Ministry of Education,Key Laboratory of Vegetable Biology of Hunan Province,Changsha 410128,Hunan,China;Institute of Vegetable and Flower,Chinese Academy of Agricultural Sciences,National Key Laboratory of Vegetable Biological Breeding,Beijing 100081,China;Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences,State Key Laboratory of Vegetable Bio-breeding,Key Laboratory of Biology and Genetics Improvement of Horticultural Crops(North China),Beijing Key Laboratory of Vegetable Germplasm Improvement,National Engineering Research Center for Vegetables,Beijing 100097,China;Changli County Agriculturai and rural Bureau,Qinhuangdao 066600,Hebei,China〕)

机构地区：[1]湖南农业大学园艺学院,园艺作物种质创新与新品种选育教育部工程研究中心,蔬菜生物学湖南省重点实验室,湖南长沙410128 [2]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [3]北京市农林科学院蔬菜研究所,蔬菜生物育种全国重点实验室,国家工程技术研究中心,蔬菜种质改良北京市重点实验室,农业农村部华北都市农业重点实验室,北京100097 [4]昌黎县农业农村局,河北秦皇岛066600

出　　处：《中国蔬菜》2023年第12期35-41,共7页China Vegetables

摘　　要：甘蓝根肿病是由芸薹根肿菌(Plasmodiophora brassicae Woronin)侵染引发的土传病害,严重影响甘蓝的生产。由于根肿菌生理小种比较复杂,因此广谱抗根肿病甘蓝材料较少。本试验以湖北长阳、重庆武隆、四川广元的根肿菌为病原菌菌源,采用孢子注射法进行接种,对抗根肿病Ogura CMS甘蓝材料GY306进行抗性鉴定。结果表明,GY306对3个地区根肿菌生理小种的抗性表现分别为免疫(DI=0)、抗病(DI=6.25)和免疫(DI=0)。同时,利用Ogura CMS育性恢复材料对GY306进行育性恢复,通过根肿病抗性鉴定筛选获得了3份抗根肿病甘蓝材料。Clubroot is a soil-borne disease caused by Plasmodiophora brassicae,which seriously threatens the production of cabbage.Due to the complex race differentiation of P.brassicae,there are fewer broad-spectrum resistance materials to clubroot.In this study,the resistance of cytoplasmic sterile clubrootresistant material‘GY306’was identified by spore injection method.The results indicated that‘GY306’was resistant to all P.brassicae from 3 different regions,and the disease grade indexes were immune(Changyang,DI=0),disease resistance(Wulong,DI=6.25)and immunity(Guangyuan,DI=0)respectively.At the same time,the fertility of‘GY306’was restored by crossing with Ogura CMS fertility recovery material.Finally,three clubroot resistance materials were obtained by identifying and screening the resistance of restored plants.

关 键 词：甘蓝 根肿病 广谱抗性 育性恢复 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]