辣椒轻斑驳病毒RT-LAMP快速检测方法的建立  

Establishment of a Rapid RT-LAMP Detection Method for Pepper Mild Mottle Virus

作　　者：邵煜尊 张万红 王惠[2] 王玉洁 苗圃[2] 申莉莉[1] 李莹 焦裕冰 杨金广[1] SHAO Yuzun;ZHANG Wanhong;WANG Hui;WANG Yujie;MIAO Pu;SHEN Lili;LI Ying;JIAO Yubing;YANG Jinguang(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory of Tobacco Pest Monitoring Controlling&Integrated Management,Qingdao 266101,Shandong,China;Luoyang Company of Henan Tobacco Company,Luoyang 471000,Henan,China)

机构地区：[1]中国农业科学院烟草研究所,烟草行业烟草病虫害监测与综合治理重点实验室,山东青岛266101 [2]河南省烟草公司洛阳市公司,河南洛阳471000

出　　处：《中国蔬菜》2023年第12期42-48,共7页China Vegetables

基　　金：青岛市科技惠民示范专项(23-2-8-xdny-12-nsh);洛阳市烟草公司科技项目(2023410300200041)。

摘　　要：辣椒轻斑驳病毒(pepper mild mottle virus,PMMo V)是发生在辣椒上的一种重要病毒病。通过逆转录环介导等温扩增技术(RT-LAMP),以PMMo V外壳蛋白CP序列为靶标基因,设计F3/B3、FIP/BIP 4条引物,构建了基于RT-LAMP技术的PMMo V快速检测方法。结果表明,该方法在60 min内便可完成对PMMo V的特异性检测,灵敏度比传统RT-PCR技术高10倍,检测过程中与其他相关病毒无交叉反应。本试验建立的PMMo V RT-LAMP检测方法操作简便、特异性强、灵敏度高,可用于田间生产中对PMMo V的快速检测。Pepper mild mottle virus(PMMoV)is a significant viral disease that occurs in peppers.In this study,we utilized reverse transcription loop-mediated isothermal amplification technology(RTLAMP)to develop a rapid PMMoV detection method.Specifically,we targeted the PMMoV coat protein CP sequence and designed four primers(F3/B3 and FIP/BIP).The results demonstrate this method enables specific detection of PMMoV within 60 minutes,offering a sensitivity 10 times higher than traditional RT-PCR technology.Furthermore,it does not exhibit any cross-reaction with other related viruses during the detection process.The established PMMoV RT-LAMP detection method is characterized by its simplicity,strong specificity,and high sensitivity,making it suitable for rapid detection of PMMoV in field production.

关 键 词：辣椒轻斑驳病毒 RT-LAMP检测 快速检测 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]