2种猪繁殖与呼吸综合征抗体ELISA检测试剂盒的比较分析  

Comparative Analysis of Two Kinds of Antibody ELISA Kit for Porcine Reproductive and Respiratory Syndrome

作　　者：王传锋[1] 米青婕 皮志媛[1] WANG Chuan-feng;MI Qing-jie;PI Zhi-yuan(Yili Vocational and Technical College,Yining,Xinjiang 835000)

机构地区：[1]伊犁职业技术学院,新疆伊宁835000

出　　处：《安徽农业科学》2023年第24期78-81,85,共5页Journal of Anhui Agricultural Sciences

基　　金：新疆维吾尔自治区科技厅地州科学基金项目(2021D01F02);伊犁州科研创新平台动物疫病检测重点实验室建设项目。

摘　　要：选取2种猪繁殖与呼吸综合征抗体ELISA检测试剂盒进行符合率、敏感性(D_(se))、特异性(D_(sp))、批内重复性(Re)比较分析,以期了解国产试剂盒与进口试剂盒在试验结果上的差异性。结果显示:通过对45份临床样品进行检测发现金诺试剂盒检测敏感性(D_(se))为84%,检测特异性(D_(sp))为100%,2种试剂盒总符合率为91.11%。该研究结果为实验室检测猪繁殖与呼吸综合征抗体提供了试剂盒选用参考。Two kinds of ELISA Kit for porcine reproductive and respiratory syndrome antibody were selected to compare the coincidence rate,sensitivity(D_(se)),specificity(D_(sp))and in-batch repeatability(Re),in order to understand the differences of experimental results between domestic and imported kits.The results showed that the detection sensitivity(D_(se))and detection specificity(D_(sp))of Jinnuo kit to 45 clinical samples were 84%and 100%,respectively.The total coincidence rate of the two kinds of kits was 91.11%.The study results provided references for the selection of laboratory kits for the detection of antibodies to porcine reproductive and respiratory syndrome.

关 键 词：猪繁殖与呼吸综合征 抗体检测 ELISA Kappa检验 比较 

分 类 号：S85[农业科学—兽医学]