谷氨酸化学交换饱和转移成像对放射性脑损伤大鼠的应用价值  

Application value of glutamate chemical exchange saturation transfer imaging in radiation-induced brain injury rat

作　　者：李国栋 刘岩 李昊[2] 刘彩云 李祥林[2] 李青龙 翁娜 宾莉 黄丹琪 王旭[1] LI Guodong;LIU Yan;LI Hao;LIU Caiyun;LI Xianglin;LI Qinglong;WENG Na;BIN Li;HUANG Danqi;WANG Xu(Department of Nuclear Medicine,Binzhou Medical University Hospital,Binzhou 256600,China;School of Medical Imaging,Binzhou Medical University,Yantai 264003,China;Department of Magenetic Resonance Imaging,Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]滨州医学院附属医院核医学科,滨州256600 [2]滨州医学院医学影像学院,烟台264003 [3]河南省中医院磁共振室,郑州450000

出　　处：《磁共振成像》2023年第12期85-90,97,共7页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金项目(编号:81771828、11805247)。

摘　　要：目的探讨7.0 T磁共振谷氨酸化学交换饱和转移(glutamate chemical exchange saturation transfer,GluCEST)成像评估放射性脑损伤大鼠模型海马谷氨酸变化的应用价值。材料与方法将实验大鼠随机分为两组:对照组(n=9)和放射性脑损伤(radiation-induced brain injury,RBI)组(n=9)。RBI组大鼠使用X射线辐照仪进行辐照,4周后进行水迷宫实验。使用7.0 T小动物磁共振采集化学交换饱和转移(chemical exchange saturation transfer,CEST)序列和高效液相色谱仪(high-performance liquid chromatography,HPLC)测量海马谷氨酸浓度。两组海马GluCEST值以及谷氨酸浓度差异进行t检验。GluCEST值与HPLC的关系、GluCEST值与水迷宫的关系进行Pearson相关性分析。结果与对照组相比,4周后RBI组大鼠认知功能受损,海马GluCEST降低(P=0.008),同样HPLC分析也证实了谷氨酸水平降低(P=0.003)。相关性分析表明HPLC测量谷氨酸浓度和GluCEST之间为正相关(r=0.71,P<0.001),空间记忆与海马GluCEST也显示出一致的正相关(r=0.50,P=0.034)。结论GluCEST成像能够可视化评估RBI大鼠海马谷氨酸变化,可用作体内代谢变化的影像生物标志物,有利于监测和干预放射治疗后伴随出现的辐射损伤。Objective:To investigate the value of 7.0 T magnetic resonance glutamate chemical exchange saturation transfer(GluCEST)imaging for assessing hippocampal glutamate changes in a rat model of radiation brain injury.Materials and Methods:The experimental rats were randomly divided into control group(n=9)and radiation-induced brain injury(RBI)group(n=9).Rats in the RBI group were irradiated using an X-ray irradiator and subjected to a water maze experiment 4 weeks later.Chemical exchange saturation transfer(CEST)sequences were collected using 7.0 T small animal magnetic resonance and hippocampal glutamate concentrations were measured by high-performance liquid chromatography(HPLC).The t-tests were performed for differences in GluCEST and glutamate concentrations in the hippocampal brain regions between the two groups.Pearson correlation analyses were performed for the relationship between GluCEST and HPLC and between GluCEST and water maze.Results:Compared to control group,rats in the RBI group had impaired cognitive function and reduced hippocampal GluCEST(P=0.008)after 4 weeks,and reduced glutamate levels were confirmed by HPLC measurements(P=0.003).Correlation analysis showed a positive correlation between glutamate concentration by HPLC and GluCEST(r=0.71,P<0.001),and spatial memory also showed a consistent positive correlation with hippocampal GluCEST(r=0.50,P=0.034).Conclusions:GluCEST imaging visualizes and evaluates hippocampal glutamate changes of RBI rats and might be used as an imaging biomarker for metabolic changes in vivo,which might be useful for the monitoring and intervention of concomitant radiation damage after radiotherapy.

关 键 词：放射性脑损伤 认知障碍 放射治疗 化学交换饱和转移成像 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]