Next-generation Sequencing of MHC Class Ⅰ Genes Reveals Trans-species Polymorphism in Eutropis multifasciata and Other Species of Scincidae  

作　　者：Shufang ZHANG Youfu LIN Yingzhi CHENG Haiyun YANG Xiaming ZHU Yu DU Longhui LIN Yanfu QU LianCHEN Hong LI 

机构地区：[1]College of Life Sciences,Nanjing Normal University,Nanjing 210023,Jiangsu,China [2]College of Life Sciences,Nanjing Forestry University,Nanjing 210037,Jiangsu,China [3]Hainan Key Laboratory of Herpetological Research,College of Fisheries and Life Science,Hainan Tropical Ocean University,Sanya 572022,Hainan,China [4]Herpetological Research Center,College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,Zhejiang,China

出　　处：《Asian Herpetological Research》2023年第4期261-270,共10页亚洲两栖爬行动物研究（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(32171495 and 31971414);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：The genes of the major histocompatibility complex(MHC) encode cell surface proteins that are essential for adaptive immunity. MHC genes show the most prominent genetic diversity in vertebrates,reflecting the adaptation of populations to their evolving environment, population survival and reproduction. In the present study, we used nextgeneration sequencing(NGS) to study the loci polymorphism of exon 3 of the MHC class Ⅰ genes in an ovoviviparous skink, the many-lined sun skink,Eutropis multifasciata and five other species of Scincidae, to quantify genetic variation. In addition,we genotyped the same MHC class Ⅰ genes of E.multifasciata using clone sequencing, to directly compare the effectiveness of both analytical techniques for MHC genotyping. NGS detected 20MHC class Ⅰ alleles in E. multifasciata, and 2 to 15 alleles in the other five Scincidae species. However,clone sequencing detected only 15 of those MHC class Ⅰ alleles in E. multifasciata. In addition, transspecies polymorphism of MHC class Ⅰ genes was studied by constructing a phylogenetic tree using the gene sequences obtained by NGS. Phylogenetic analysis revealed that MHC class I alleles were shared among different species of Scincidae with trans-species polymorphism, and did not exhibit specific genealogical inheritance. These results have important implications for understanding polymorphism interspecies diversity in the MHC genes of Scincidae, and the evolution of the MHC more broadly.

关 键 词：Eutropis multifasciata major histocompatibility complex next-generation sequencing SCINCIDAE trans-species polymorphism 

分 类 号：Q953[生物学—动物学]