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作 者:夏华美 曹志坚 于铭玥 李珍[1] 聂春雅 张鹏阳 郭欢 XIA Huamei;CAO Zhijian;YU Mingyue;LI Zhen;NIE Chunya;ZHANG Pengyang;GUO Huan(College of Grassland Agriculture,Northwest A&F University,Yangling 712100,Shaanxi,China;Yinchuan Rural Economic Development Service Center,Yinchuan 753000,Ningxia,China)
机构地区:[1]西北农林科技大学草业与草原学院,陕西杨凌712100 [2]银川市农村经济发展服务中心,宁夏银川753000
出 处:《草业科学》2023年第12期3124-3137,共14页Pratacultural Science
基 金:陕西省重点研发计划一般项目(2023-YBNY-046);国家自然科学基金项目(32101253);西北农林科技大学博士科研启动金项目(2452020228)。
摘 要:为了发掘耐盐性强的优良草种质资源,本研究对常见的30份商用草地早熟禾(Poa pratensis)品种苗期的耐盐性进行鉴定评价。在400 mmol·L-1 NaCl胁迫下,测定了草地早熟禾的株高、分蘖数、生物量、叶绿素含量和叶片相对含水量等一系列生长和生理指标,并采用主成分分析法对草地早熟禾耐盐性进行综合评价。结果显示:在盐胁迫下,不同品种草地早熟禾的生长均受到抑制。其中‘肯塔基’的株高和地上部鲜重受盐胁迫的影响最小,与无盐对照相比,分别下降了32.62%和18.14%,而‘蓝之梦’地上部生物量受盐胁迫的影响最大,其地上部鲜重和干重分别较对照下降了71.80%和53.29%;与对照相比,盐处理后‘肯塔基’‘优美’‘月光’‘探险家’和‘乐土’的叶绿素含量下降幅度较低,均下降了20%上下,‘蓝精灵’叶绿素b含量下降高达71.40%,‘满月’的总叶绿素含量下降了55.70%。盐胁迫下,‘蓝精灵’‘满月’和‘优异’的叶片相对含水量下降幅度较大,超过了22%。结合主成分分析综合得分,耐盐性较强的草地早熟禾品种有‘肯塔基’‘乐土’‘探险家’‘月光’和‘优美’,而‘蓝精灵’‘飞达’‘优异’‘蓝之梦’和‘满月’对盐胁迫较为敏感。To explore the genetic resources of Kentucky bluegrass(Poa pratensis)with strong salt tolerance,we evaluated the salt tolerance of 30 commercial Kentucky bluegrass varieties at the seedling stage.A range of growth and physiological indicators including plant height,tiller number,biomass,chlorophyll content,and leaf relative water content were determined under 400 mmol·L−1 NaCl stress.A comprehensive evaluation was carried out using principal component analysis.The results indicate that salt stress inhibited the growth of all Kentucky bluegrass varieties.Compared to the control(without salt treatment),plant height and shoot fresh weight of‘Kentucky’decreased by 32.62%and 18.14%,respectively,after salt treatment.‘Blue Best’was the most severely impacted variety,with a 71.80%reduction in shoot fresh weight and dry weight decreased by and a 53.29%reduction in shoot dry weight due to salt stress.Furthermore,the chlorophyll content of‘Kentucky’‘Euromyth’‘Moonlight’‘Explorer’and‘Arcadia’decreased by approximately 20%following salt treatment.‘Blue Coat’experienced a notable 71.40%decrease in chlorophyll content,while the total chlorophyll content of‘Fullmoon’decreased by 55.70%.Additionally,under salt treatment,the leaf relative water content of‘Blue Coat’‘Fullmoon’and‘Merit’decreased by more than 22%compared to the control.Based on the comprehensive evaluation scores for salt tolerance,‘Kentucky’‘Arcadia’‘Explorer’‘Moonlight’and‘Euromyth’demonstrated strong salt tolerance,whereas‘Blue Coat’‘Fielder’‘Merit’‘Blue Best’and‘Fullmoon’proved to be sensitive to salt stress.
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