利用BSA-Seq技术初步鉴定棉花芽黄候选基因  

Preliminary Identification of Candidate Genes withVirscent Gene in Cotton Based on BSA-Seq

作　　者：王娟[1] 王新[1] 周小凤[1] 马晓梅[1] 田琴[1] 李保成[1] 余渝[1] 董承光[1] WANG Juan;WANG Xin;ZHOU Xiao-feng;MA Xiao-mei;TIAN Qin;LI Bao-cheng;YU Yu;DONG Cheng-guang(Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs,Shihezi 832000,Xinjiang)

机构地区：[1]新疆农垦科学院/农业农村部西北内陆区棉花生物学与遗传育种重点实验室,新疆石河子832000

出　　处：《绿洲农业科学与工程》2023年第2期25-29,共5页Oasis Agriculture Science and Engineering

基　　金：农业生物育种重大专项(2023ZD04040-5);兵团科技创新人才计划(2022CB003-04);第六师五家渠市科技计划项目(2205)。

摘　　要：芽黄性状可作为棉花育种的标记性状,在棉花杂交选育中可减轻棉花杂交制种和鉴定过程,还可以作为叶绿素等生理代谢研究的理想材料。研究棉花芽黄突变体具有重要的理论和实践意义。本研究以芽黄突变体115-23与绿叶正常材料JSH1527为亲本,构建F2分离群体,鉴定后代芽黄性状,选择30个芽黄单株和30个正常绿叶单株构建混合池,对4个样本(2个亲本池和2个混合池)开展全基因组重测序,采用SNP-index进行关联分析,确定芽黄基因的候选区域,筛选芽黄候选基因。结果表明,在2个亲本之间共获得840,612个多态性SNP,利用Δ(SNP-index)方法,在95%的置信区间内将候选区域定位到染色体D02区间,包含93个候选基因,其中4个基因功能注释与叶绿体合成等相关,可能是调控棉花叶色的候选基因。研究结果可为棉花芽黄分子机制及芽黄相关基因的克隆奠定理论基础。Virescent trait could be used as a natural marker for breeding to reduce time and process of hybrid breeding,moreover they were ideal model systems to study chlorophyll and chloroplast biosynthesis and biodegradation process.the virescent mutant in cot⁃ton is of great theoretical and practical implications.In this study,an F2 segregation population was constructed derived from 115-23(virscent mutant)and JSH1527(Normal green trait),based on the identification of virescent trait,30 virescent and 30 green plants were selected to construct a mixed pool,four samples(2 parent pools and 2 mixed pools)were sequenced,SNP-index were used for association analysis to determine the candidate regions of virescentgenes.As a result,840,612 SNPs were obtained between the two parents,one candidate physical regions showing confidence indices higher than 95%were obtained on chromosome D02,which con⁃tained 93 annotated genes,four of these genes were functional annotations related to chloroplast synthesis and may be candidate genes for the regulation of cotton leaf color.This study laid a foundation for further analyzing the genetic mechanism and clones of genes of cotton virescent formation.

关 键 词：棉花 芽黄 BSA-Seq 候选基因 

分 类 号：S51[农业科学—作物学]