高强度间歇性运动前后高水平女子足球运动员尿液代谢组学特征研究  

Urinary Metabolomic Profile of High-Level Female Soccer Players at before and after High-Intensity Interval Training

作　　者：赵薇[1] 王长权[2] 侯莉娟[2] ZHAO Wei;WANG Changquan;HOU Lijuan(Northwest Normal University,Lanzhou 730070,China;Beijing Normal University,Beijing 100875,China)

机构地区：[1]西北师范大学,甘肃兰州730070 [2]北京师范大学,北京100875

出　　处：《体育科学》2023年第7期74-82,共9页China Sport Science

基　　金：教育部人文社会科学研究规划基金(21YJA890031)。

摘　　要：目的:采用液相色谱-质谱联用(LC-MS)的代谢组学方法,探究高水平女子足球运动员在一场比赛前后能量代谢调节的变化情况,旨在为女子足球运动员机能评定与训练监控提供理论依据。方法:选取参加2021年大学生女子超冠联赛半决赛的10名运动员作为实验对象,分别采集运动员比赛当天晨尿、赛前30 min和赛后30 min的尿样。所有数据经过转换、峰检测、归一化处理后再进行多元统计分析。结果:高强度间歇性运动前后高水平女子足球运动员尿样代谢物色谱图发生了显著变化。与赛前相比,赛后的乳酸、肌酸、丙酮酸、肌酐、L-缬氨酸、色氨酸、丙氨酸等代谢物显著上调(VIP>1.3且P<0.01),腺嘌呤、β-葡萄糖、酪氨酸、柠檬酸、琥珀酸等代谢物显著下降(VIP>1.4且P<0.01)。结论:1)高水平女子足球运动员在大强度比赛后显著变化的代谢物主要涉及ATP-CP系统、糖酵解系统、有氧氧化系统、神经调节系统以及氨基酸代谢系统;2)代谢组学方法能够为运动员在备战期和赛前的饮食与营养补充以及运动效果的评价提供更全面、更具针对性的数据分析与指导。Objective:To investigate the changes of energy metabolism regulation in high-level female football players before and after a match through the liquid chromatography-mass spectrometry(LC-MS),so as to provide theoretical reference for the assessment of female football players'performance and monitoring the training.Methods:10 female athletes who participated in the semi-finals of the 2021 College Women's Super Champion League were participated in this study.The urine samples in the morning,30 minutes before and 30 minutes after the competition were collected.Data were converted,peak detected,and normalized for multivariate statistical analysis.Results:Significant differences in urine metabolite chromatograms were observed at before and after intermittent high-intensity interval training.Compared with before exercise,metabolites including lactate,creatine,pyruvate,creatinine,L-valine,tryptophan and alanine were significantly up-regulated after exercise(VIP>1.3 and P<0.01),the adenine,β-glucose,tyrosine,citric acid and succinic acid were decreased significantly(VIP>1.4 and P<0.01).Conclusions:1)The metabolic changes observed in high-level female soccer players after intense matches mainly involve the ATP-CP system,glycolysis system,aerobic oxidation system,neural regulation system,and amino acid metabolism system;2)Metabolomics methods can provide more comprehensive and targeted data analysis and guidance for athletes'diet and nutrition supplementation during preparation and pre-competition period as well as evaluation of their performance.

关 键 词：高强度间歇性运动 女子足球运动员 比赛 代谢组学 

分 类 号：G804.7[文化科学—运动人体科学]