野蔷薇花瓣转录组分析  

Transcriptome Analysis and Differential Gene Screening at Different Opening Stages of Rose multiflora Thunb.Petals

作　　者：熊燕[1] 陈菲[1] 李黎[1] 付俊生 韩辉[1] 曲彦婷[1] XIONG Yan;CHEN Fei;LI Li;FU Jun-sheng;HAN Hui;QU Yan-ting(Institute of Natural Resources and Ecology,HAS,Harbin,Heilongjiang 150040)

机构地区：[1]黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040

出　　处：《辽宁农业科学》2023年第6期6-11,共6页Liaoning Agricultural Sciences

基　　金：黑龙江省科学院科学研究基金(KY2021ZR05);黑龙江省省属科研院所科研业务费项目(CZKYF2021-2-C008);黑龙江省科学院双提雁阵特别计划(STY2022ZR01);黑龙江省科学院人才队伍建设平台项目(RC2022ZR01)。

摘　　要：为了揭示野蔷薇(Rose multiflora Thunb.)花瓣开放过程中花瓣扩展相关基因的表达情况,试验以野蔷薇为材料,通过RNA-seq技术分析不同开放阶段的花瓣中差异基因的表达,为进一步深入研究野蔷薇花瓣转录水平上的发育调控提供依据。结果表明:转录组分析组装得到52865个单基因(Unigenes),通过对NR数据库的比对,功能注释匹配物种中,与野蔷薇匹配的物种前3位为野草莓(Fragaria vesca subsp.vesca)(36.17%)、粳稻(Oryza sativa Japonica)(13.65%)、棉花(Gossypium hirsutum)(4.04%)。对花瓣开放的3个比较组(FB_CP vs FB_GP,FB_YP vs FB_CP,OF_WP vs FB_YP)的差异基因比较,各阶段的差异基因分别为642、902、1899个。通过对不同开花阶段花瓣扩展相关基因表达分析,可能受EXP,XTH基因家族及细胞壁合成、修饰和水解相关基因XYL,CES的作用。This experiment aimed to investigate the gene expression related to petal expansion during the opening of Rose multiflora petals.To achieve this,RNA-seq analysis was performed on petals at various stages of opening.The results of this study provide basis for further exploration of the transcriptional regulation of Rose multiflora petal development.The results indicated that:The transcriptome analysis resulted in the assembly of 52865 Unigenes.These were functionally annotated by comparing them to the NR database,and were found to match with various species.The top three species that matched were Fragaria vesca subsp.vesca(36.17%),Oryza sativa Japonica(13.65%),and Gossypium hirsutum(4.04%).A total of 642902 and 1899 differential genes were identified in the three stages of petal opening(FB_CPvsFB_GP,FB_YPvsFB_CP,OF_WPvsFB_YP).By analyzing the expression of genes related to petal expansion at different flowering stages.These genes may be influenced by EXP,XTH gene family,XYL,and CES genes related to cell wall synthesis,modification,and hydrolysis.

关 键 词：野蔷薇 转录组 花朵开放 花瓣扩展 

分 类 号：S685.120.1[农业科学—观赏园艺]