Therapeutic Effect of Daphnetin on Mastitis Induced by Staphylococcus aureus in Mice  

作　　者：Yi LI Qianjiong HUANG Jinhui JIANG Guoyang LIN Chenchen HUANG Jie GAO 

机构地区：[1]Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China [2]Department of Pathology and Pathophysiology,Youjiang Medical University for Nationalities,Baise 533000,China

出　　处：《Medicinal Plant》2023年第6期60-63,共4页药用植物：英文版

基　　金：Supported by Project of Innovation and Entrepreneurship Training Program for College Students in Guangxi Zhuang Autonomous Region in 2022(S202210599060);Basic Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Zhuang Autonomous Region in 2020(2020KY13027);Project of Scientific Research and Technological Development Plan of Baise City in 2020(Encyclopedia[2020]No.47).

摘　　要：[Objectives]To observe the effects of daphnetin on mastitis induced by Staphylococcus aureus in mice.[Methods]18 postpartum ICR female mice were used to establish mastitis animal model,and were randomly divided into three groups(A,B,and C)with 6 mice in each group.Group A:blank control group;group B:S.Aureus model group;group C:S.Aureus model+daphnetin group.The experimental groups were injected 1 mL of 1.0×104 CFU/100μL of S.aureus of along the nipple catheter.The suspension was placed in the 3 rd and 4 th pairs of mammary glands,and the control group was injected with the same dose of normal saline.On the second day after infection,the rats in group A,B and C were given drugs by gavage,while the rats in group A and B were given normal saline and the rats in group C were given daphnetin once a day for 6 consecutive days.Blood samples were collected from living eyeballs,and blood cells were analyzed by automatic flow cytometer after anticoagulation.[Results]The NLR and Systemie Immune Inflammati-on Index(SII)in the blood of mastitis mice induced by S.aureus were significantly higher than those in the control group(P<0.01),suggesting that neutrophil to lymphocyte ratio(NLR)and SII can be used as diagnostic indicators of mastitis,and the levels of NLR and SII decreased significantly after daphnetin intervention.[Conclusions]NLR and SII showed high levels in mastitis mice,which are valuable for the diagnosis of mastitis and the evaluation of its prognosis.After the intervention of daphnetin,both of them decreased significantly,indicating that daphnetin has a good prognosis trend in mastitis mice induced by S.aureus.

关 键 词：DAPHNETIN MASTITIS Staphylococcus aureus Red cell distribution width(RDW) Neutrophil to lymphocyte ratio(NLR) Ratio of platelets to lymph-ocytes(PLR) Systemie Immune Inflammati-on Index(SII) 

分 类 号：R285.5[医药卫生—中药学]