2015年~2021年我国东部地区猪流行性腹泻病毒S1蛋白关键表位的变异分析  

Variation analysis of epitopes for S1 protein of porcine epidemic diarrhea virus in Eastern China from 2015 to 2021

作　　者：程亚豪 胡灵子 刘雅琦 周莹珊 杜静 周兴东 王晓杜 吴瑗[2] 董婉玉 CHENG Ya-hao;HU Ling-zi;LIU Ya-qi;ZHOU Ying-shan;DU Jing;ZHOU Xing-dong;WANG Xiao-du;WU Yuan;DONG Wan-yu(College of Animal Science and Technology&College of Veterinary Medicine,Zhejiang A&F University,Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province,Provincial Engineering Research Center for Animal Health Diagnostics&Advanced Technology,Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management,China Australia Joint Laboratory for Animal Health Big Data Analytics,Hangzhou 311300,China;Faculty of Agriculture,Jinhua Polytechnic,Jinhua 321007,China)

机构地区：[1]浙江农林大学动物科技学院/动物医学院,浙江省畜禽绿色生态健康养殖应用技术研究重点实验室/动物健康互联网检测技术浙江省工程实验室/动物医学与健康管理浙江省国际科技合作基地/中澳动物健康大数据分析联合实验室,浙江杭州311300 [2]金华职业技术学院农学院,浙江金华321007

出　　处：《中国预防兽医学报》2023年第9期968-972,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31972656);浙江省农业重大技术协同推广项目(2021XTTGXM02-05);浙江省三农六方项目(2021SNLF023);杭州市农业与社会发展科研项目(20201203B61);金华市农业重点研发项目(2022-2-022);浙江农林大学科研训练项目(S202210341099)。

摘　　要：为了解2015年~2021年我国东部地区猪流行性腹泻病毒(PEDV)的流行特征和遗传变异规律,本研究利用RT-PCR扩增2015年~2017年11株分离自浙江地区PEDV流行株的S1基因序列,并与GenBank中我国东部地区(上海、江苏、山东、河南和安徽)的86株PEDV流行株的S1基因序列通过MEGA 7.0软件比较分析S1基因的遗传进化关系,采用CLC Sequence Viewer 8软件分析比对S1蛋白氨基酸序列的变异。PEDV S1基因遗传进化分析结果显示,97株PEDV中有92%的流行株属于G2型,与疫苗株CV777同源性较低(90.4%~96.1%),山东地区2017年~2018年间的部分流行株与疫苗株CV777等属于G1型;G2型中,33%的流行株属于G2a型,67%的流行株属于G2b亚型,其中2019年~2021年的流行株有15%流行株属于G2a亚型,85%的流行株属于G2b亚型。S1蛋白关键表位的变异分析结果显示,与经典株CV777相比,中和表位COE(CO-26K equivalent epitope)发生8个氨基酸位点(A^(517)S、S^(523)G、V^(527)I、T^(549)S、G^(594)S、A^(605)E/D、L^(612)F/Y、I^(635)V)的突变,SS2区域无氨基酸变异,SS6发生1个氨基酸位点(Y^(766)S)的突变,2018年后出现新的突变位点(T^(636)I和S^(749)G)。不同地区S1蛋白关键表位的变异分析结果显示,aa501变异在河南地区(L^(501)P)和江苏地区(L^(501)W)存在明显的差异;aa536突变(F^(536)L)多集中于河南、山东地区的流行株,aa542突变(D^(542)E)集中于江苏、上海地区。本研究首次系统分析了我国东部地区PEDV流行株S1基因及其氨基酸的遗传变异规律,为我国该地区PEDV变异株疫苗选择、生物安全防控方案制定提供了参考依据。In order to analyse the epidemic characteristics and the genetic variation of porcine epidemic diarrhea virus(PEDV)in Eastern region of China from 2015 to 2021,the S1 gene sequence of 11 PEDV epidemic strains in Zhejiang during 2015-2017 were obtained by RT-PCR and the resulting sequences were used to compared with another S1 gene sequences of 86 PEDV stains in Eastern region of China(Shanghai,Jiangsu,Shandong,Henan and Anhui Provinces)available in GenBank.MEGA 7.0 software was used for analysis of the genetic evolution relationship among S1 gene above.CLC Sequence Viewer 8 software was used for the amino acid sequence of S1 protein.The results of genetic evolution analysis for the S1 gene showed that 92%of the epidemic strains belonged to the G2 group and revealed low levels of sequence homology with vaccine strains CV777 sequences(90.4%-96.1%).Some of the epidemic strains during 2017-2018 in Shandong Province and vaccine strains CV777 belonged to the G1 group.In the G2 group,33%of the epidemic strains belonged to the G2a subgroup,67%of the epidemic strains belonged to the G2b subgroup.15%of the epidemic strains in 2019-2021 belonged to the G2a subgroup,and 85%belonged to the G2b subgroup.Analysis of variation for the key epitopes of the S1 protein showed that compared with the classical strain CV777,8 amino acid mutations(A^(517)S,S523G,V^(527)I,T^(549)S,G^(594)S,A^(605)E/D,L^(612)F/Y,I^(635)V)in the neutralization epitope COE(CO-26K equivalent epitope)were mutated.The SS6 epitope showed 1 amino acid site mutations(Y766S).And new mutation sites emerged since 2018(T^(636)I and S^(749)G).The variation analysis of key epitopes of S1 protein in different regions showed that the variation of amino acid 501 was significantly different between Henan(L^(501)P)and Jiangsu(L^(501)W).The amino acid mutation at site 536(F^(536)L)was concentrated in the endemic strains in Henan and Shandong,and the amino acid mutation at site 542(D542E)was concentrated in Jiangsu and Shanghai.This study is the first time to analyze systemat

关 键 词：猪流行性腹泻病毒 S1基因 遗传变异 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]