蛋白质印迹羧化多壁碳纳米管/CaAlg水凝胶膜及其修饰的电化学传感器  

Protein Molecularly Imprinted Carboxylated Multi-Walled Carbon Nanotubes/CaAlg Hydrogel Film and Its Modified Electrochemical Sensor

作　　者：高欣悦 郭智龙 董鹏 谢慧珂 张世潮 江坚盛 赵孔银[1] 侯彦辉[1] Xinyue Gao;Zhilong Guo;Peng Dong;Huike Xie;Shichao Zhang;Jiansheng Jiang;Kongyin Zhao;Yanhui Hou(School of Materials Science and Engineering,Tiangong University,Tianjin 300387,China)

机构地区：[1]天津工业大学材料科学与工程学院,天津300387

出　　处：《高分子材料科学与工程》2023年第12期111-117,共7页Polymer Materials Science & Engineering

基　　金：大学生创新创业训练计划(202010058089,202010058065)。

摘　　要：文中以牛血清白蛋白(BSA)为模板、海藻酸钠(NaAlg)为功能单体,掺杂羧化多壁碳纳米管,经CaCl2溶液交联制备了BSA分子印迹羧化多壁碳纳米管/海藻酸钙(CMWCNTs/CaAlg)复合水凝胶膜(MIP),同时不用模板制备了非印迹膜(NIP),研究了CMWCNTs含量对膜力学性能的影响;膜厚度、洗脱液pH值对BSA吸附性能的影响。研究了MIP膜的吸附选择性。结果表明,当CMWCNTs的质量为NaAlg的2%时,水凝胶膜同时具备良好的抗溶胀性能和力学性能。膜厚度控制在0.2mm,洗脱液pH值为7.4时,MIP膜对BSA的吸附量最大,为35.04mg/g。将MIP膜负载于裸碳电极表面,循环伏安法和差分脉冲伏安法测试表明,MIP膜修饰电极对BSA表现出较高的选择识别性。The BSA molecularly imprinted carboxylated multi-walled carbon nanotubes(CMWCNTs)hydrogel composite films were prepared by crosslinking with CaCl2 solution,using bovine serum albumin(BSA)as template and natural macromolecule sodium alginate(NaAlg)as functional monomer,while the non-imprinted film(NIP)was prepared without template.The effects of CMWCNTs content on the mechanical properties of the films and the effects of film thickness and pH value of eluents on the adsorption properties of BSA were studied.The selective adsorption of MIP film was studied by comparing the adsorption capacity of MIP films to different proteins.The results show that when the mass fraction of CMWCNTs is 2%of NaAlg mass,the hydrogel film has good swelling and mechanical properties.When the film thickness is 0.2 mm and the pH value of the eluent is 7.4,the adsorption effect of the hydrogel film on BSA is the best,reaching 35.04 mg/g.The MIP is loaded on the surface of bare carbon,and the performance of the modified electrode was measured by cyclic voltammetry(CV)and differential pulse voltammetry(DPV).The results show that compared with NIP modified electrode,the MIP modified electrode shows a higher selective recognition to BSA.

关 键 词：蛋白质分子印迹 海藻酸钙水凝胶 羧化多壁碳纳米管 电化学传感器 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]