新疆阿拉尔市流浪猫源蜱种类鉴定及其携带立克次体PCR检测  

Identification of Tick Species of Stray Cat Origin and PCR Detection for Rickettsia Carried by Them in Alar City,Xinjiang Uygur Autonomous Region

作　　者：司俊飞 赫永强 张振杰 朱婷婷 张旭 曹梦雅 赵爱云[1] 齐萌[1] Si Junfei;He Yongqiang;Zhang Zhenjie;Zhu Tingting;Zhang Xu;Cao Mengya;Zhao Aiyun;Qi Meng(College of Animal Science and Technology,Tarim University,Alar,Xinjiang 843300 China;Institute of Agricultural Sciences(Institute of Animal Science),9th Division of Xinjiang Production andConstruction Corps,Emin,Xinjiang 834601,China)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]新疆生产建设兵团第九师农业科学研究所(畜牧科学研究所),新疆额敏834601

出　　处：《中国动物检疫》2023年第12期22-27,共6页China Animal Health Inspection

基　　金：新疆生产建设兵团第九师科技计划项目(2023JS002)。

摘　　要：为了解猫体表寄生蜱的种类及其携带立克次体情况,从新疆阿拉尔市某居民小区流浪猫体表采集蜱样本,基于形态学和分子生物学方法鉴定蜱种类,然后采用PCR法检测其立克次体携带情况,通过序列比对鉴定其基因型,并构建遗传进化树分析其分子遗传特征。结果显示:4只流浪猫中有1只体表寄生了3只蜱,通过形态学观察初步鉴定为图兰扇头蜱;基于蜱线粒体SSU rRNA和COX I基因对3只蜱DNA样本进行PCR扩增,经序列分析比对为图兰扇头蜱;基于立克次体OmpA和OmpB基因,分别对3只蜱DNA样本进行PCR扩增和序列比对分析发现,在2只蜱DNA样本中检测到的立克次体序列与我国图兰扇头蜱和黎巴嫩具环扇头蜱源暂定巴布瑞立克次体同源性为100%,将其鉴定为暂定巴布瑞立克次体。结果表明,阿拉尔市流浪猫体表存在图兰扇头蜱寄生,其可携带暂定巴布瑞立克次体。本研究结果为我国猫源寄生蜱及其传播病原学研究提供了基础资料。In order to investigate tick species on the body surface of cats and Rickettsia carried by them,tick samples were collected from stray cats in a residential area of Alar,Xinjiang to identify tick species using the morphological and molecular biology method,and to detect the Rickettsia carried by ticks using PCR.Then the genotype of Rickettsia was identified through sequence matching,and a genetic evolution tree was constructed to analyze its molecular genetic characteristics.The results revealed that one of the 4 stray cats had 3 ticks parasitized on its body surface,which were preliminarily identified as Rhipicephalus turanicus through morphological observation;based on the mitochondrial SSU rRNA and COXⅠgenes,the DNA samples of the three ticks were amplified by PCR,and the ticks were finally identified as Rhipicephalus turanicus.Based on OmpA and OmpB genes of Rickettsia,the DNA samples of the three ticks were amplified by PCR and their sequences were compared and analyzed,it was found that the Rickettsia sequence detected in the DNA of two ticks was 100%homologous to the Candidatus R.barbariae from Rhipicephalus turanicus in China and Rhipicephalus annulatus in Lebanon,hence the Rickettsia was identified as Candidatus R.barbariae.In conclusion,there were Rhipicephalus turanicus parasitized on the body surface of stray cats in Alar,which could carry Candidatus R.barbariae.A basic data was thus provided for researches on parasitic ticks of cat origin and their pathogen carrying status in China.

关 键 词：图兰扇头蜱 斑点热群立克次体 流浪猫 暂定巴布瑞立克次体 

分 类 号：S851.3[农业科学—预防兽医学]