假蒟根中一个新的酰胺类生物碱  

A new amide alkaloid from the root of Piper sarmentosum

作　　者：邵金华 徐拓 吕敬崑 陈小明 SHAO Jin-hua;XU Tuo;LYU Jing-kun;CHEN Xiao-ming(School of Chemistry and Bioengineering,Hunan University of Science and Engineering;Hunan Provincial Engineering Research Center for Ginkgo Folium,Hunan University of Science and Engineering;Hunan Hengwei Pharmaceutical Co.,Ltd.,Yongzhou 425100,China)

机构地区：[1]湖南科技学院化学与生物工程学院 [2]湖南省银杏工程技术研究中心 [3]湖南恒伟药业股份有限公司,永州425100

出　　处：《天然产物研究与开发》2023年第12期2082-2087,共6页Natural Product Research and Development

基　　金：湖南省自然科学基金(2020JJ9026)。

摘　　要：研究假蒟(Piper sarmentosum)根的化学成分及其抗炎活性,采用硅胶、Sephadex LH-20等柱色谱和半制备HPLC等色谱分离技术,从假蒟根的95%乙醇提取物中分离得到10个生物碱类成分,结合其理化性质和波谱数据,分别鉴定为chaplupyrrolidone D(1)、magnolamide(2)、N-反式桂皮酰对羟基苯乙胺(3)、N-反式对香豆酰酪胺(4)、吲哚-3-甲醛(5)、吲哚-3-甲酸(6)、N-苯甲酰基-2-苯基乙胺(7)、N-苯甲酰酪胺(8)、N-benzoyltyramine methyl ether(9)、荷蒂芸香酰胺(10)。其中,化合物1为新的酰胺类生物碱,除化合物5外其他化合物均为首次从假蒟中分离得到。抗炎活性测试结果表明,化合物1、2、4和10对LPS诱导RAW 264.7巨噬细胞产生NO具有不同程度的抑制作用,IC 50值分别为34.65±1.78、25.41±0.94、15.27±0.82和39.57±2.10μmol/L。The chemical composition of Piper sarmentosum roots and their anti-inflammatory activity were investigated.A new amide alkaloid,namely chaplupyrrolidone D(1),together with nine known compounds,namely magnolamide(2),N-trans-cinnamoyltyramine(3),trans-N-p-coumaroyltyramine(4),indole-3-carbaldehyde(5),indole-3-carboxylic acid(6),N-benzoyl-2-phenylethylamine(7),N-benzoyltyramine(8),N-benzoyltyramine methyl ether(9),hortiamide(10),was isolated and purified from the 95%ethanol extract of P.sarmentosum roots by comprehensive chromatography methods of silica gel column,Sephadex LH-20 gel column and semi-preparative HPLC.All compounds except compound 5 are obtained from P.sarmentosum for the first time.Biologically,compounds 1,2,4 and 10 exhibited inhibitory effects on LPS-induced NO production in RAW 264.7 cells with IC 50 values of 34.65±1.78,25.41±0.94,15.27±0.82 and 39.57±2.10μmol/L,respectively.

关 键 词：假蒟 化学成分 生物碱 抗炎活性 

分 类 号：R284.1[医药卫生—中药学]