基于转录组测序挖掘槐山羊高繁关键候选基因  

Identification of Differentially Expressed Genes in Ovary Tissue of Huai Goats with Different Lambing Numbers Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing

作　　者：韩浩园 李世凯 杨瑞巧 李曼曼 李君 哈斯 赵金艳 魏红芳 权凯 HAN Haoyuan;LI Shikai;YANG Ruiqiao;LI Manman;LI Jun;HA Si;ZHAO Jinyan;WEI Hongfang;QUAN Kai(College of Animal Science and Technology,Henan University of Animal Husbandry and Economics,Zhengzhou 450046,China;College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]河南牧业经济学院动物科技学院,郑州450046 [2]华南农业大学动物科学学院,广州510642

出　　处：《畜牧兽医学报》2023年第12期5077-5090,共14页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：河南省重点研发与推广专项(科技攻关)(222102110024);河南省现代农业产业技术体系建设专项资金(HARS-22-15-S)。

摘　　要：为了探究山羊繁殖性状的内在分子调控机制以及基因调控网络。本研究选取健康无病的3岁左右、体格大小相似的第3胎次母羊,依据繁殖性能记录,采集3只平均胎产羔数为1只的母羊为单羔组,采集3只平均胎产羔数大于2只的母羊为多羔组。采用转录组测序技术进行mRNA文库构建、单羔组与多羔组间差异表达基因筛选及功能鉴定,通过实时荧光定量PCR技术验证基因表达量。本研究构建了6个槐山羊卵巢组织mRNA文库,平均reads总数为41 163 239条,clean reads数为37 908 182条。差异表达分析在多羔与单羔组间共发现121个差异表达基因(P<0.05),其中上调基因30个,下调基因91个。PTX3、MMP13、PAK1、ADAMTS1、COL1A2、CCN1、SLC4A10、FOS、NR4A1、NR4A2和ADCY8等11个基因在单羔与多羔组间表达差异显著(P<0.05),且参与组织器官结构发育功能及内分泌系统醛固酮、皮质醇、催产素和促肾上腺皮质激素等激素分泌;在上述11个基因中共发现236个SNPs,其中7个SNPs为错义突变,4个位于ADAMTS1基因,1个位于PTX3基因,2个位于CCN1基因。本研究结果表明,11个基因在单羔组与多羔组间表达量差异显著,且参与组织器官结构发育及内分泌系统的激素分泌,可能与山羊卵泡发育、排卵及类固醇激素分泌有关,为阐明槐山羊多胎性状遗传机制提供科学依据。The study aimed to explore molecular regulatory mechanism and genes regulatory network of goat reproductive traits.In this study,transcriptome sequencing was used to explore gene expression and regulatory networks in ovarian tissues with different lambing numbers.In this study,3-year-old healthy ewes of 3rd parity with similar body size were selected.According to the reproductive performance records,3 ewes with 1 lamb on average were collected as single-lamb group,and 3 ewes with more than 2 lambs on average were collected as multi-lamb group.Transcriptome sequencing technology was used to construct mRNA library,screen differentially expressed genes(DEGs)and conduct functional enrichment analysis between single-lamb group and multi-lamb group.Real-time fluorescence quantitative PCR was used to verify gene expression.Six mRNA libraries of ovarian tissues from Huai goats were constructed,with a total number of 41163239 raw reads and 37908182 clean reads on average.A total of 121 DEGs were found between single-lamb group and multi-lamb group(P value<0.05),including 30 up-regulated genes and 91 down-regulated genes.The expressions of PTX3,MMP13,PAK1,ADAMTS1,COL 1A2,CCN1,SLC4 A 10,FOS,NR 4A1,NR 4A2 and ADCY 8 genes were significantly differentially expressed between single-lamb group and multi-lamb group(P value<0.05).And these 11 DEGs were involved in the animal organs development and the secretion of aldosterone,cortisol,oxytocin and adrenocorticotropic hormone in the endocrine system.A total of 236 SNPs were screened in the above 11 DEGs.Seven SNPs were nonsynonymous mutations,among which,4 SNPs were detected in ADAMTS 1 gene,one was in PTX 3 gene,and two were in CCN 1 gene.The results showed that the expression of 11 genes were significantly different between single-lamb group and multi-lamb group.These DEGs were involved tissues and organs development and hormone secretion in endocrine system.These DEGs may be related to goat follicular development,ovulation and steroid hormone secretion.The results will provide

关 键 词：槐山羊 产羔数 转录组 差异表达基因 

分 类 号：S826.2[农业科学—畜牧学]