HPLC测定姜黄鲜切饮片中的姜黄素类成分  

Determination of curcumin class content of freshly cut decoction pieces of Curcumae LongaeRhizoma

作　　者：袁茂华 万子玉[1] 瞿雅懿 余敏灵 杨玉英 刘航 YUAN Maohua;WAN Ziyu;QU Yayi;YU Minling;YANG Yuying;LIU Hang(The Key Laboratory of Quality Research in Chinese Medicine of Sichuan Medical Products Administration,Leshan Food and Drug Inspection and Testing Center,Leshan,Sichuan,614000 P.R.China;State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of TCM,Chengdu,Sichuan,611137 P.R.China)

机构地区：[1]乐山市食品药品检验检测中心四川省药品监督管理局中药质量研究重点实验室,四川乐山614000 [2]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137

出　　处：《华西药学杂志》2023年第6期674-677,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川药品监督管理局科技计划项目(2021012);乐山市科技局重点研究项目(22ZDYJ0109,22ZDYJ0106)。

摘　　要：目的以乙醇-水为洗脱系统来测定姜黄鲜切饮片中姜黄素类成分的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙醇-水(41:59),流速0.75 mL·min^(-1),柱温35℃,检测波长430 nm。结果姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的线性范围为32.4~486.0、6.2~93.0、17.8~267.0μg·mL^(-1)(r^(2)均≥0.9998);平均回收率为100.97%~103.39%,RSD为0.96%~1.69%;供试品溶液在24 h内稳定,RSD为0.15%~0.83%(n=7)。11批样品中姜黄素、去甲氧基姜黄、双去甲氧基姜黄素的含量分别为1.76%~2.49%、0.53%~0.76%、0.43%~0.63%。结论所用方法结果可靠、环保、准确、重复性好、回收率高,可用于姜黄鲜切饮片的含量测定。OBJECTIVE To determine the content of curcumin class in freshly cut decoction pieces of Curcumae Longae Rhizoma by HPLC with ethanol-water.METHODS The determination was performed on a Kromasil C_(18)(250 mm×4.6 mm,5μm)column with a mobile phase gradient elution of ethanol-water(41:59).The flow rate was 0.75 mL·min~(-1),column temperature was 35℃,and the detection wavelength was 430 nm.RESULTS A good linear relationship was obtained between the peak areas and the concentrations of curcumin,desmethoxycurcumin and bisdesmethoxycurcumin in the range of 32.4-486.0μg·mL^(-1),6.2-93.0μg·mL^(-1),and 17.8-267.0μg·mL^(-1).The average recovery was in the range of 100.97%-103.39%,with the RSD of 0.96%-1.69%.The test solution was stable within 24 h with the RSD of 0.15%-0.83%(n=7).The content of curcumin,desmethoxyturmeric and bisdesethoxycurcumin in 11 batches of samples were 1.76%-2.49%,0.53%-0.76%,and 0.43%-0.63%,respectively.CONCLUSION The method is reliable,environmentally friendly,accurate,and repeatable with high recovery,which can be used for determination of curcumin class content in freshly cut decoction pieces of Curcumae Longae Rhizoma.

关 键 词：姜黄药材 姜黄 鲜切饮片 姜黄素 去甲氧基姜黄素 双去甲氧基姜黄素 高效液相色谱法 乙醇 含量测定 

分 类 号：R917[医药卫生—药物分析学]