石南藤与混淆品小叶爬崖香的鉴别研究  

Identification of Piper wallichii and its confusable variety Piper sintenense

作　　者：胡玉洁 杜富强 谢军丽 龚小见[4] 李玲 马江雄 李栋 汤瑾 黄健 范东生 HU Yujie;DU Fuqiang;XIE Junli;GONG Xiaojian;LI Ling;MA Jiangxiong;LI Dong;TANG Jin;HUANG Jian;FAN Dongsheng(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Department of Pharmacy,the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China;Guizhou Warmen Pharmaceutical Co.Ltd.,Guiyang 550018,China;Guizhou Key Laboratory for Information System and Protection of Ecological Environment of Mountainous Areas,Guizhou Normal University,Guiyang 550001,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州中医药大学第一附属医院药学部,贵州贵阳550001 [3]贵州威门药业股份有限公司,贵州贵阳550018 [4]贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001

出　　处：《贵州科学》2023年第6期14-18,共5页Guizhou Science

基　　金：国家自然科学基金地区基金(81960787);贵州省科技计划项目(No.黔科合LH字〔2017〕7146号);贵州省“十四五”中医药、民族医药重点学科建设项目[No.QZYYZDXK(JS)-2021-03];博士科研启动基金(GYZYYFY-BS-2018【14】);贵州省2018年度高层次创新型人才项目;贵州省中药、民族医药科学技术研究课题(QZYY2017-079和087)。

摘　　要：目的:对石南藤和混淆品小叶爬崖香进行鉴别研究。方法:采用石蜡切片法、粉末临时制片法和薄层鉴别法,从显微特征及化学成分方面研究石南藤与小叶爬崖香的差异性。结果:石南藤茎横切片中髓维管束较少,大小较均匀,小叶爬崖香髓维管束较多,大小差异明显;石南藤茎粉末中淀粉为单粒或复粒,非腺毛为多细胞,而小叶爬崖香中淀粉多为单粒,非腺毛主要为单细胞;以futoquinol为对照品的薄层色谱鉴别方法能有效的区分石南藤与小叶爬崖香。结论:石南藤与小叶爬崖香显微结构和化学成分存在差异,期望本研究为二者的快速鉴别提供一定的科学依据。This study aims at distinguishing Piper wallichii from its confusable variety Piper sintenense.The differences in microscopic characteristics and chemical composition between P.wallichii and P.sintenense were investigated by using paraffin sectioning,powder provisional sectioning and thin-layer chromatography identification.The results showed that the medullary bundles in the transverse section of the stem of P.wallichii were small in number and uniform in size,whereas they were more numerous in P.sintenense,with a difference in size.The starch in the stem powder of P.wallichii was single or compound granular,and the nonglandular hairs were multicellular,whereas the starch in P.sintenense was mostly single granular,and the nonglandular hairs were unicellular.The two varieties were efficiently distinguished via the thin-layer chromatographic method with futoquinol as the control.In conclusion,there are differences in microstructure and chemical composition between P.wallichii and P.sintenense.This study has provided a scientific basis for the rapid identification of P.wallichii and P.sintenense.

关 键 词：石南藤 小叶爬崖香 显微结构鉴别 薄层色谱鉴别 

分 类 号：R284.2[医药卫生—中药学]