槐角中染料木苷和槐角苷的匀浆-超声协同提取工艺的研究  

Homogenate-Ultrasonic Synergistic Extraction of Genistin and Sophoricoside from Styphnolobium japonicum Pods

作　　者：范昌文 李欣 徐明[3] 杨磊[1] 付卓锐[3] 莫开林[3] FAN Changwen;LI Xin;XU Ming;YANG Lei;FU Zhuorui;MO Kailin(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin150040,China;Liangshan Forestry Investigation,Planning and Design Institute,Xichang 615000,China;Sichuan Academy of Forestry,Chengdu 610081,China)

机构地区：[1]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [2]凉山州林业调查规划设计院,四川西昌615000 [3]四川省林业科学研究院,四川成都610081

出　　处：《四川林业科技》2023年第6期105-109,共5页Journal of Sichuan Forestry Science and Technology

基　　金：黑龙江省自然科学基金项目(C2018005)。

摘　　要：以槐角为原料,乙醇作为提取溶剂,利用匀浆-超声协同辅助的方法提取染料木苷与槐角苷。采用高效液相色谱法为定量手段,考察了乙醇体积分数、液料比、匀浆时间、匀浆速率、超声时间、超声功率、超声空化时间和超声缓冲时间这些因素对染料木苷和槐角苷得率的影响。确定最佳提取条件分别为:乙醇体积分数50%,液料比20 mL·g^(−1),匀浆时间3 min,匀浆速率22000 r·min^(−1),超声时间35 min,超声功率350 W,超声空化时间2.5 s,超声缓冲时间1.5 s。匀浆-超声协同提取法与传统提取方法相比,具有高效、环保、节能的优点。Using the Styphnolobium japonicum pods as raw materials and ethanol as extraction solvent,genistin and sophoricoside were extracted by homogenate-ultrasonic synergistic extraction method.Effects of ethanol volume fraction,liquid-solid ratio,homogenate time,homogenate speed,ultrasonic time,ultrasonic power,cavitation time and buffer time on the yield of genistin and sophoricoside were investigated by the quantitative method of high performance liquid chromatography.The best extraction conditions were as follows:ethanol volume fraction of 50%,liquid-solid ratio of 20 mL·g^(−1),homogenate time of 3 min,homogenate speed of 22000 r·min^(−1),ultrasonic time of 35 min,ultrasonic power of 350 W,cavitation time of 2.5 s and buffer time of 1.5 s.Compared with the traditional extraction method,the homogenate ultrasonic-synergistic extraction method had the advantages of high efficiency,environmental protection and energy saving.

关 键 词：槐角 匀浆-超声协同提取 染料木苷 槐角苷 

分 类 号：R284.2[医药卫生—中药学]