甘蓝型油菜GLK基因家族全基因组鉴定及耐盐抗旱功能分析  

Identification of GLK gene family and expression patterns under salt and drought stress in Brassica napus

作　　者：邹雨婷 刘丹 王秋艳 夏柔 汪文倩 杨敏[1] 童雅微 危文亮 ZOU Yu-ting;LIU Dan;WANG Qiu-yan;XIA Rou;WANG Wen-qian;YANG Min;TONG Ya-wei;WEI Wen-liang(College of Agriculture,Yangtze University/MARA Key Laboratory of Sustainable Crop Production in the MiddleReaches of the Yangtze River,Jingzhou 434025,China)

机构地区：[1]长江大学农学院/农业农村部长江中游作物绿色高效生产重点实验室,湖北荆州434025

出　　处：《中国油料作物学报》2023年第6期1156-1165,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31871661)。

摘　　要：GLK(GOLDEN2-like)转录因子是GARP转录因子超家族的成员,可直接激活大量光合作用相关蛋白的靶基因,包括叶绿素生物合成、光捕获和电子传输相关的靶基因,在植物生理活动过程和非生物胁迫应激反应中发挥关键作用。为解析甘蓝型油菜GLK基因家族,探究其与油菜响应盐旱胁迫的相关功能,本研究在甘蓝型油菜全基因组水平上鉴定出159个BnaGLK基因,对其进行了基因结构特征、系统进化、启动子顺式作用元件、基因表达模式等分析。结果表明,159个BnaGLK基因不均匀地分布在19条染色体上。它们可被分为9个亚族,每个亚族中BnaGLK基因的外显子和内含子数量和结构都非常相似。启动子分析发现了多种激素和非生物胁迫响应相关的顺式调控元件,特别是干旱响应元件。选择在油菜叶片中高表达的8个BnaGLKs进行了盐旱胁迫下的表达模式分析。结果揭示BnaGLK基因在油菜非生物胁迫响应中发挥重要作用。本研究为进一步解析GLK基因家族在甘蓝型油菜中的生物学功能奠定了基础。The GLK(the Golden2-like)transcription factor,a member of the GARP transcription factor super-family,can directly activate a large number of downstream target genes encoding photosynthesis-related proteins,including the target genes associated to chlorophyll biosynthesis,light capture and electron transport,and play a key role in plant physiological processes and abiotic stress responses.In order to identify GLK gene family in Brassica napus and explore their related functions in response to salt and drought stress,159 BnaGLK genes were identified on the whole genome level,and their gene structure characteristics,phylogenetic evolution,promoter cis-acting ele-ments and gene expression patterns were analyzed.The results showed that 159 BnaGLK genes were unevenly dis-tributed on 19 chromosomes.They were divided into 9 subgroups,and the number and structure of exons and in-trons of the BnaGLK gene were very similar in each subgroup.Promoter analysis revealed a number of cis-acting el-ements,including a variety of hormonal and abiotic stress responsive,particularly drought responsive cis-acting ele-ments.Eight BnaGLKs with high expression in leaves based on the open-accessed expression data,were selected for further expression pattern analysis under salt and drought stress by qRT-PCR.The results indicated that Bna-GLK genes play an important role in abiotic stress response in B.napus.This study laid a foundation for further analysis of the biological function of GLK gene family in B.napus.

关 键 词：油菜 GLK基因家族 全基因组分析 干旱胁迫 盐胁迫 表达模式 

分 类 号：S565.4[农业科学—作物学]