支原体肺炎并发脑炎患儿血清和脑脊液中巨噬细胞炎症蛋白-1α和人软骨糖蛋白39水平变化及临床意义  

The change and clinical significance of macrophange inflammtory protein-1α and human cartilage glycoprotein 39 levels in serum and cerebrospinal fluid of children with mycoplasma pneumonia complicated with encephalitis

作　　者：李韶军 简维国 刘新宇 刘迪程 朱月 Li Shaojun;Jian Weiguo;Liu Xinyu;Liu Dicheng;Zhu Yue(Department of Clinical Laboratory,Shishou People's Hospital,Shishou 434400,China)

机构地区：[1]石首市人民医院检验科,石首434400

出　　处：《中国医师进修杂志》2023年第12期1099-1103,共5页Chinese Journal of Postgraduates of Medicine

摘　　要：目的探讨支原体肺炎并发脑炎患儿血清和脑脊液中巨噬细胞炎症蛋白-1α(MIP-1α)和人软骨糖蛋白39(YKL-40)水平变化及临床意义。方法回顾性选取2018年7月至2020年5月石首市人民医院收治的108例支原体肺炎患儿为研究对象,其中并发脑炎组66例,未并发脑炎组42例;选取53例同期参加体检的健康儿童为正常对照组。采用酶联免疫吸附法检测各组血清和脑脊液中MIP-1α、YKL-40水平;相关性分析采用Pearson检验;采用受试者工作特征(ROC)曲线评价血清和脑脊液中MIP-1α和YKL-40水平对支原体肺炎并发脑炎的诊断价值。结果并发脑炎组和未并发脑炎组血清MIP-1α和YKL-40水平均高于正常对照组[(84.82±12.85)、(63.46±9.23)mg/L比(24.12±2.45)mg/L,(53.46±9.25)、(31.45±7.35)ng/L比(23.95±6.38)ng/L],且并发脑炎组血清MIP-1α和YKL-40水平高于未并发脑炎组,差异有统计学意义(P<0.05);并发脑炎组脑脊液中MIP-1α和YKL-40水平高于未并发脑炎组[(124.82±20.85)mg/L比(113.46±18.23)mg/L、(46.46±10.25)ng/L比(22.95±5.38)ng/L],差异有统计学意义(P<0.05)。Pearson检验结果显示,支原体肺炎患儿血清MIP-1α、YKL-40水平呈正相关(r=0.697,P<0.05),脑脊液中MIP-1α、YKL-40水平呈正相关(r=0.403,P<0.05)。ROC曲线分析结果显示,血清YKL-40、MIP-1α水平预测支原体肺炎并发脑炎患儿的曲线下面积(AUC)分别为0.845、0.885,二者联合预测支原体肺炎并发脑炎患儿的AUC为0.943,高于单一指标检测水平。结论支原体肺炎患儿血清及脑脊液中MIP-1α和YKL-40水平升高,二者联合检测可作为预测支原体肺炎患儿并发脑炎的血清标志物。Objective To investigate the change and clinical significance of macrophange inflammtory protein-1α(MIP-1α)and human cartilage glycoprotein 39(YKL-40)levels in serum and cerebrospinal fluid of children with mycoplasma pneumonia complicated with encephalitis.Methods A total of 108 children with mycoplasma pneumonia treated in Shishou People's Hospital from July 2018 to May 2020 were retrospectively selected as the study objects,including 66 patients with encephalitis and 42 patients without encephalitis.Fifty-three healthy children who participated in physical examination during the same period were selected as the normal control group.The levels of MIP-1αand YKL-40 in serum and cerebrospinal fluid were detected by enzyme-linked immunosorbent assay(ELISA).Pearson test was used for correlation analysis.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of MIP-1αand YKL-40 levels in serum and cerebrospinal fluid for mycoplasma pneumonia complicated with encephalitis.Results The serum levels of MIP-1αand YKL-40 in the encephalitis group and without encephalitis group were higher than those in the normal control group:(84.82±12.85)and(63.46±9.23)mg/L vs.(24.12±2.45)mg/L,(53.46±9.25)and(31.45±7.35)ng/L vs.(23.95±6.38)ng/L,and the serum levels of MIP-1αand YKL-40 in the encephalitis group were higher than those in the without encephalitis group,there were statistical differences(P<0.05).The cerebrospinal fluid levels of MIP-1αand YKL-40 in the encephalitis group were higher than those in the without encephalitis group:(124.82±20.85)mg/L vs.(113.46±18.23)mg/L,(46.46±10.25)ng/L vs.(22.95±5.38)ng/L,there were statistical differences(P<0.05).The results of Pearson test showed that the levels of MIP-1αand YKL-40 in serum and cerebrospinal fluid had positively correlated(r=0.697,0.403,P<0.05).ROC curve analysis results showed that the area under the curve(AUC)of serum YKL-40 and MIP-1αlevels in the diagnosis of children with mycoplasma pneumonia complicated with encephalit

关 键 词：肺炎 支原体 脑炎 巨噬细胞炎症蛋白-1Α 人软骨糖蛋白39 预后 

分 类 号：R725.6[医药卫生—儿科] R742.9[医药卫生—临床医学]