Insertion of unnatural metal ligand in the heme pocket of nitrophorin through protein semi-synthesis:Toward biomimicking binuclear active sites  

作　　者：Yonglu Chen Yi Zhou Chunmao He 

机构地区：[1]School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510640,China

出　　处：《Chinese Chemical Letters》2023年第12期132-135,共4页中国化学快报（英文版）

基　　金：financial support from the National Natural Science Foundation of China(Nos.22077036 and 22277029)is greatly acknowledged.

摘　　要：Herein,we report a semi-synthetic strategy affording a nitrophorin 2(NP2)variant with a N,N'-bis(2-pyridylmethyl)amine(Dpa)ligand as sidechain selectively installed at position 27,which was assembled from a synthetic peptide thioester bearing the Dpa ligand and an expressed protein segment via native chemical ligation.The semi-synthetic NP2 was able to accept the natural heme b cofactor and the Dpa ligand was able to bind Cu(Ⅱ)/Fe(Ⅲ)ions,leading to heteronuclear active site.

关 键 词：Protein semi-synthesis Protein modification Nitrophorin2 Heteronuclear active site Native chemical ligation 

分 类 号：O629.73[理学—有机化学]