In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment  

作　　者：Haocheng Gong Yanhui Zhang Ying Gao Xinrong Tian Pengzhao Wu Xiaoyu Wei Yuan Guo 

机构地区：[1]Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education,College of Chemistry and Materials Science,Northwest University,Xi'an710127,China

出　　处：《Chinese Chemical Letters》2023年第12期452-455,共4页中国化学快报（英文版）

基　　金：National Natural Science Foundation of China(Nos.21977082,22037002 and 21472148);the Natural Science Basic Research Program of Shaanxi(No.2020JC-38).

摘　　要：We here present a Förster resonance energy transfer(FRET)-based and environment-sensitive fluorescent probe VG-1 for vicinal-dithiol-containing proteins(VDPS).VG-1 uniquely contalns two sites sensitive to the protein environment(SPE)thus it shows weak fluorescence in both blue and green channels(a low FRET efficiency)in solution.After specifically binding with VDPs,its fuorescence in the green channel increases,while that in the blue channel disappears,achieving the specific detection of VDPs.The obvioussignal changes in fluorescence may be attributed to that the increased rigidity of the molecular skeletons causes the enhanced FRET eficiency,The probe also achleved the cell super-resolution imaging of VDPs and the confocal imaging of VDPs in zebrafish.

关 键 词：Vicinal-dithiol-containing proteins Environment sensitivity Super-resolution imaging Fluorescent probes Forster resonance energy transfer 

分 类 号：Q503[生物学—生物化学] O657.3[理学—分析化学]