针刺治疗膝关节骨性关节炎的效果及对低氧诱导因子-1α表达的影响  

The effect of acupuncture treatment on knee osteoarthritis and its influence on the expression of hypoxia-inducible factor-1α

作　　者：王红辉[1] Wang Honghui(Department of Orthopedic,the First Affiliated Hospital of Nanyang Medical Unversity,Nanyang 473000,China)

机构地区：[1]南阳医学高等专科学校第一附属医院骨二科,南阳473000

出　　处：《中华实验外科杂志》2023年第11期2200-2203,共4页Chinese Journal of Experimental Surgery

摘　　要：目的:观察针刺治疗膝关节骨性关节炎的效果及对低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)表达的影响。方法:30只SD大鼠根据随机数字表法随机分为对照组、模型组和针刺组,每组10只,模型组和针刺组采用木瓜蛋白酶膝关节注射建模。建模成功后,对照组和模型组不做处理,针刺组采用无菌电疗仪每天连续治疗20 min。5次为一个疗程,治疗2个疗程。采用Mankin’s病理评分法评估关节软骨病理损伤程度;采用行为观测系统分析大鼠关节活动程度;采用酶联免疫吸附实验(ELISA)分析大鼠关节滑液炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和血VEGF水平;采用蛋白质免疫印迹分析大鼠关节组织HIF-1α表达水平。组间数据比较采用单因素方差分析。结果:针刺组大鼠Mankin’s病理评分[(4.10±1.45)分]明显低于模型组[(8.90±1.20)分],差异有统计学意义(t=8.075,P<0.05)。针刺组大鼠关节活动度[(119.24±9.48)°]明显高于模型组[(92.39±9.15)°],差异有统计学意义(t=6.444,P<0.05)。针刺组大鼠关节肿胀率[(112.15±1.87)%]明显低于模型组[(23.92±3.56)%],差异有统计学意义(t=9.251,P<0.05)。针刺组大鼠关节滑液TNF-α和IL-6表达水平[(83.02±9.74)、(53.79±7.70)μg/ml]明显低于模型组[(130.21±17.64)、(91.98±6.56)μg/ml],差异有统计学意义(t=3.551、11.940,P<0.05)。针刺组大鼠滑膜液VEGF水平[(169.39±21.13)μg/ml]明显高于模型组[(92.39±9.15)μg/ml],差异有统计学意义(t=11.150,P<0.05)。针刺组大鼠关节活动度[(1.31±0.10)°]明显低于模型组[(1.01±0.11)°],差异有统计学意义(t=5.778,P<0.05)。结论:针刺能够有效缓解骨性关节炎大鼠膝关节炎性反应,改善膝关节的功能,可能与HIF-1α表达水平下调有关。Objective The effect of acupuncture treatment on knee osteoarthritis and its impact on hypoxia inducible factor-1α(HIF-1α)expression.Methods Thirty SD rats were randomly divided into a control group,a model group,and an acupuncture group using a random number table method,with 10 rats in each group.The model group and the acupuncture group were modeled by injecting papain into the knee joint.After successful modeling,control group and model group were left untreated,while the acupuncture group received continuous treatment with a sterile electrotherapy device for 20 minutes per day.5 times is a course of treatment,with 2 courses of treatment.Evaluate the degree of pathological damage to articular cartilage using the Mankin’s pathological scoring method.The degree of joint activity in rats was analyzed by a behavioral observation system.The levels of inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and vascular endothelial growth factor(VEGF)in rat synovial fluid was analyzed by enzyme linked immunosorbent assay(ELISA).The expression level of HIF-1αin rat joint tissue was analyzed by Protein immunoblotting was used to analyze.Single factor analysis of variance was used for inter group data comparison.Results The pathological score of Mankin’s disease in the acupuncture group rats(4.10±1.45)was significantly lower than that in the model group rats(8.90±1.20,t=8.075,P<0.05).The joint swelling degree of the acupuncture group rats[(119.24±9.48)°]was significantly higher than that of the model group rats[(92.39±9.15)°,t=6.444,P<0.05].The joint activity of the acupuncture group rats[(112.15±1.87)%]was significantly lower than that of the model group rats[(23.92±3.56)%,t=9.251,P<0.05].Acupuncture group rat joint synovial fluid TNF-αand IL-6 expression levels[(83.02±9.74),(53.79±7.70)μg/ml]significantly lower than the model group rats[(130.21±17.64),(91.98±6.56)μg/ml,t=3.551,11.940,P<0.05].The level of VEGF in synovial fluid of rats in the acupuncture group[(169.39±21.13)μg

关 键 词：针刺 膝关节骨性关节炎 低氧诱导因子-1Α 

分 类 号：R245[医药卫生—针灸推拿学]