大鼠原位肝移植术后腹腔细菌感染对移植物免疫排斥反应的影响  

Effects of graft acute rejection after intraabdominal bacterial infection following orthotopic liver transplantation in rats

作　　者：林拥华[1] 陈志超 吴敏[1] 魏剑锋[1] Lin Yonghua;Chen Zhichao;Wu Min;Wei Jianfeng(Department of Hepatobiliary and Pancreatic Surgery,the Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China)

机构地区：[1]福建医科大学附属第二医院肝胆胰外科,福建医科大学第二临床学院,泉州362000

出　　处：《中华实验外科杂志》2023年第11期2245-2248,共4页Chinese Journal of Experimental Surgery

摘　　要：目的:探讨大鼠原位肝移植术后腹腔细菌感染对移植肝排斥反应的影响。方法:采用成年雄性DA、Lewis大鼠(上海斯莱克实验动物有限责任公司)为供受体建立大鼠原位肝移植模型,采用随机数字表分组法分成4组,G1为LEW到LEW,同基因对照组;G2为LEW到LEW,同基因腹腔感染组;G3为DA到LEW,异基因对照组;G4为DA到LEW,异基因腹腔感染组。每组于术后第2、4、6、8天4个时间点(每个时间点4只)收集标本,通过观察移植肝病理表现、细胞凋亡,评估移植肝排斥反应的不同表现。组间比较采用单因素方差分析,病理等级资料采用秩和检验。结果:苏木精-伊红(HE)显示,感染后第5天(术后第8天),G3组RAI高于G4组(8.0±0.8比6.5±1.3,χ^(2)=12.711,P<0.05)。感染后5 d(术后第8天)G4组移植肝实质及汇管区凋亡细胞指数(AI)高于G3组[(28.53±5.27)%比(17.33±4.99)%,F=32.298,P<0.05]。结论:大鼠肝移植术后腹腔细菌感染诱导移植肝汇管区及小叶中央静脉周围浸润淋巴细胞凋亡增多,导致移植肝排斥反应减轻。Objective To discuss the effects of graft acute rejection after intraabdominal bacterial infection following orthotopic liver transplantation in rats.Methods The intraabdominal bacterial infection three days after rat orthotopic liver transplantation model was developed according with previous method.Which were randomly divided into four groups:G1(LEW→LEW,non-infection group),G2(LEW→LEW,infection group),G3(DA→LEW,non-infection group),G4(DA→LEW,infection group).Histopathology and cell apoptosis of grafts were observed at four time points,i.e.2,4,6,8 d after surgery.One-way analysis of variance was used for comparison between groups,and rank sum test was used for pathological grade data.Results RAI(Rejection activity index)varied significantly between G3 and G4(8.0±0.8 vs.6.5±1.3,χ^(2)=12.711,P<0.05)5 d after infection(i.e.8 d after operation).The apoptosis index(AI)of apoptotic cells in the sink area and hepatic parenchyma varied significantly between G4 and G3[(28.53±5.27)%vs.(17.33±4.99)%,F=32.298,P<0.05]5 d after infection(i.e.8 d after operation).Conclusion Intraabdominal bacterial infection induced the increase of apoptosis of infiltrated lymphocytes in the sink area and around the central lobular vein of grafts after ROLT,which led to the alleviating of the acute rejection.

关 键 词：肝移植 大鼠 急性排斥反应 腹腔细菌感染 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学]