甘草次酸差向异构体的HPLC检测方法研究  

Establishment of an HPLC Detection Method for the Distinction of Glycyrrhetinic Acid Isomers

作　　者：张博冉 武秋颖[1] 相林 惠连旺 刘会娟 许可 ZHANG Bo-ran;WU Qiu-ying;XIANG Lin;HUI Lian-wang;LIU Hui-juan;XU Ke(Department of Life Sciences,Tangshan Normal University,Tangshan063000,China;Maybarke(Hebei)Pharmaceutical Co.,Ltd.,Tangshan063000,China;Institute of Biochemical Engineering,Department of Chemical Engineering,School of Chemistry and Chemical Engineering,Beijing Institute of Technology,Beijing 100081,China)

机构地区：[1]唐山师范学院生命科学系,河北唐山063000 [2]迈巴克(河北)药业有限公司,河北唐山063000 [3]北京理工大学化学与化工学院,北京100081

出　　处：《唐山师范学院学报》2023年第6期46-48,82,共4页Journal of Tangshan Normal University

基　　金：河北省省级科技计划(21374301D,B2022105015);唐山市科技计划(21130201C);唐山师范学院科研基金项目(2022B09)。

摘　　要：使用液相色谱型号为Agilent 1260 Infinity II;C18反相色谱柱,4.6 mm×250 mm,粒径5μm;检测波长254 nm,柱温30℃,进样量10μL;流动相甲醇和10 mmol/L高氯酸水溶液(氨水调pH=8.2,体积比为78∶22 v/v),流速1.0 mL/min。在上述条件下进样测定,18α-GA和18β-GA分离度较好,并且能够稳定检测压力,保留时间分别为11.321 min和12.865 min;线性范围为10~100 mg/L,重复性实验RSD依次为0.5%和0.3%。The liquid chromatography was model Agilent1260 Infinity II and the detection conditions were as follows:C18 reverse phase chromatographic column,4.6mm×250mm,particle size5μm;detection wavelength of 254nm,column temperature of 30℃,injection volume of 10μL,mobile phase of methanol:10mmol/L hydrochloric acid aqueous solution(ammonia adjusted pH=8.2)(78∶22v/v),flow rate of 1.0mL/min.Under the above conditions,18α-GA and18β-GA were well separated and stable detection pressure was achieved,with retention times of 11.321min and 12.865min,respectively.The linear range was10~100mg/L,and the RSD of the repeatability experiment was 0.5%and 0.3%,respectively.

关 键 词：18α-甘草次酸 18Β-甘草次酸 差向异构体 HPLC检测 

分 类 号：R927.2[医药卫生—药学]