盐胁迫对苍术幼苗生理指标的影响  

Effects of Salt Stress on Physiological Indexes of Atractylodes Lancea Seedlings

作　　者：曹佳诺 王佳佳 项竞仪 陈桂平[1] CAO Jia-nuo;WANG Jia-jia;XIANG Jing-yi;CHEN Gui-ping(Department of Life Sciences,Tangshan Normal University,Tangshan 063000,China;College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,China;College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100091,China)

机构地区：[1]唐山师范学院生命科学系,河北唐山063000 [2]河北师范大学生命科学学院,河北石家庄050024 [3]北京林业大学生物科学与技术学院,北京100091

出　　处：《唐山师范学院学报》2023年第6期49-53,共5页Journal of Tangshan Normal University

基　　金：河北省科技厅项目(21327509D);唐山市科技计划项目(20150201C);唐山市重点试验室(2020TS004b)。

摘　　要：以苍术幼苗为材料,检测0.6%、0.9%NaCl胁迫0 h、12 h、24 h、48 h后根部丙二醛含量等8种生理指标的变化,来解析苍术的耐盐生理机制。结果表明,盐胁迫下,丙二醛含量先上升后下降;脯氨酸含量、可溶性糖含量的变化均呈上升趋势,且与CK组相比变化显著(P<0.05);可溶性蛋白含量呈现先升高后降低再升高的趋势;相对电导率呈上升趋势,且与CK组相比变化极显著(P<0.01);SOD、POD活性都呈现先降低后升高再降低的趋势,POD活性相比CK组变化显著(P<0.05);两个胁迫组CAT活性变化不同,0.6%胁迫组呈现先降低后升高的趋势,0.9%胁迫组呈现先降低后升高再降低的趋势。Using Atractylodes macrocephala seedlings as materials,the changes in eight physiological indicators,including the content of malondialdehyde in the roots,were detected after 0,12,24,and 48 hours of NaCl stress at 0.6%and 0.9%,in order to elucidate the physiological mechanism of salt tolerance.The results showed that the content of malondialdehyde first increased and then decreased;the content of proline and soluble sugar increased in the stress group,and the change was significant compared with CK Group(P<0.05);the content of soluble protein increased firstly,then decreased and then increased;the relative conductivity also increased,and the change was very significant compared with CK Group(P<0.01);the activity of SOD and POD decreased first,then increased and then decreased;the activity of POD changed significantly compared with CK Group(P<0.05).The changes of CAT activity in the two stress groups were different.The CAT activity in the 0.6%stress group decreased first and then increased,while that in the 0.9%stress group decreased first,then increased and then decreased.

关 键 词：苍术 盐胁迫 保护酶 可溶性糖 相对电导率 丙二醛含量 

分 类 号：Q945.78[生物学—植物学]