合成树脂类材料的细胞毒性方法差异性分析  

Differential Analysis of Cytotoxicity Methods for Synthetic Resin Materials

作　　者：钟文雨 黄虹蓉 杨安然 孔怡然 林钟石 ZHONG Wenyu;HUANG Hongrong;YANG Anran;KONG Yiran;LIN Zhongshi(Shenzhen Testing Center of Medical Divices,Shenzhen 518000,China)

机构地区：[1]深圳市医疗器械检测中心,广东深圳518000

出　　处：《化工管理》2023年第36期50-52,共3页Chemical Engineering Management

基　　金：广东省药品监督管理局科技创新项目“生物源性医疗器械病毒灭活验证方法及标准研究科技创新团队”(2022TDB53)。

摘　　要：以PE材料、PP材料、PS材料、PVC材料为合成树脂类材料的代表,采用中性红摄取法、集落形成法、MTT比色法、XTT比色法这四种定量试验方法测定其细胞毒性,比较不同机理的试验方法对材料的细胞毒性结果的影响。试验结果表明,合成树脂类材料采用不同机理的试验方法测得的细胞毒性结果无显著性差异,可以为合成树脂类材料的医疗器械选择合适的细胞毒性试验方案提供实验依据。PE material,PP material,PS material,PVC material as the representative of synthetic resin materials,using neutral red uptake method,colony formation method,MTT colorimetry,XTT colorimetry four quantitative test methods to determine their cytotoxicity,compared the test methods of the different mechanism on the cytotoxicity results of the material.The results showed that there was no significant difference between the cytotoxicity of synthetic resin materials measured by test methods with different mechanisms.The results provide experimental basis for selection of appropriate cytotoxicity test scheme for medical devices made of synthetic resin materials.

关 键 词：医疗器械 合成树脂 中性红 集落形成 细胞毒性 

分 类 号：TQ322[化学工程—合成树脂塑料工业]