复方蟾酥胶囊对小鼠Lewis肺癌移植瘤的药效学研究  

Efficacy of Compound Toad Venom Capsules on Xenograft Tumor in Lewis Lung Cancer Mice

作　　者：毕莉斯 谢春祥 赵荣华 刘树民[1] BI Lisi;XIE Chunxiang;ZHAO Ronghua;LIU Shumin(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang Chanbao Biotechnology Development Co.,Ltd.,Hegang 154100,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江蟾宝生物科技发展有限公司,黑龙江鹤岗154100

出　　处：《中医药信息》2023年第12期8-13,共6页Information on Traditional Chinese Medicine

基　　金：黑龙江中医药大学优秀创新人才(领军人才)基金项目(2018RCL13)。

摘　　要：目的:探讨不同剂量复方蟾酥胶囊对C57BL/6J小鼠Lewis肺癌的抑制作用。方法:选取60只SPF级雄性C57BL/6J小鼠,随机分为空白组10只和造模组50只。空白组单独饲养,造模组采用小鼠Lewis肺癌细胞株(LLC)进行肺癌肿瘤右侧腋窝皮下造模,造模成功后随机分为模型组、顺铂组和复方蟾酥胶囊低、中、高剂量组,每组各10只。顺铂组隔日腹腔注射顺铂,复方蟾酥胶囊低、中、高剂量组每天用不同剂量复方蟾酥胶囊溶液[0.1365、0.273、0.546 g/(kg·d)]灌胃,空白组不予处理;模型组灌胃生理盐水,14 d后处死小鼠并取出肿瘤组织及肺组织。采用ELISA法、实时荧光定量PCR、蛋白免疫印迹法、HE染色检测小鼠肿瘤组织中肿瘤坏死因子-α(TNF-α)含量,半胱氨酸蛋白酶(Caspase-3)、血管内皮生长因子(VEGF)mRNA表达、蛋白表达及肺组织病理情况来测定肿瘤抑制情况。结果:与模型组比较,复方蟾酥胶囊中剂量组及顺铂组瘤重显著降低(P<0.05),TNF-α水平降低(P<0.05,P<0.01),显著上调Caspase-3 mRNA和蛋白的表达水平(P<0.05,P<0.01,P<0.0001),显著下调VEGF mRNA和蛋白的表达水平(P<0.01,P<0.001);模型组肺组织中多见肺泡壁增厚,伴有较多淋巴细胞与中性粒细胞浸润,局部支气管可见上皮细胞坏死,核碎裂,有明显肺水肿及出血,顺铂组和复方蟾酥胶囊各剂量组能明显改善上述情况,且肺泡腔内有嗜酸性浆液样物质渗出。结论:复方蟾酥胶囊具有抑制Lewis肺癌瘤体生长的作用。Objective:To investigate the inhibitory effect of different doses of Compound Toad Venom Capsules on Lewis lung cancer in C57BL/6J mice.Methods:Sixty SPF male C57BL/6J mice were selected and randomly divided into blank group(n=10)and model group(n=50).Blank group was raised separately.Mouse Lewis lung cancer cell line(LLC)was used for subcutaneous modeling of the right axilla of lung cancer tumor in the modeling group.After successful modeling,the group was randomly sub-divided into model group,cisplatin group and compound toad venom capsule low-dose,medium-dose and high-dose groups,with 10 mice in each group.The cisplatin group was intraperitoneally injected with cisplatin every other day,the low,medium and high dose groups of Compound Toad Venom Capsules were given different doses of compound toad venom capsule solution[0.1365,0.273,0.546 g/(kg·d)]every day,and the blank group was not treated.The model group was given physiological saline by gavage,and after 14 days,the mice were euthanized and tumor tissue and lung tissue were removed.The content of tumor necrosis fact-α(TNF-α),mRNA expression and protein expression of Caspase-3,vascular endothelial growth factor(VEGF)and lung pathology were detected by ELISA,real-time fluorescent quantitative PCR,Western blot and HE staining,to measure tumor suppression.Results:Compared with model group,the tumors in the medium dose group of compound toad venom capsule and the cisplatin group were significantly reduced(P<0.05),TNF alpha levels decreased(P<0.05,P<0.01),Caspase-3 mRNA and protein expression levels were up-regulated significantly(P<0.05,P<0.01,P<0.0001),VEGF mRNA and protein expression levels were significantly down-regulated(P<0.01,P<0.001).Alveolar wall thickening was observed in the model group,accompanied by more lymphocyte and neutrophil infiltration.Epithelial cell necrosis,nuclear fragmentation,pulmonary edema and hemorrhage were observed in local bronchus.Cisplatin group and compound toad venom capsule dosage groups can significantly improve the above

关 键 词：复方蟾酥胶囊 LEWIS肺癌 TNF-Α CASPASE-3 VEGF 

分 类 号：R285.5[医药卫生—中药学]