文氏马齿苋汤对急性湿疹小鼠血清IgE、IL-2和IL-5的影响  

The Effects of Wenshi Purslane Decoction on Serum IgE,IL-2 and IL-5 in Mice with Acute Eczema

作　　者：邹瑾 景明夷 朱宝华 陈明岭[1,2] ZOU Jin;JING Mingyi;ZHU Baohua;CHEN Mingling(Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610000,China;Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528400,China)

机构地区：[1]成都中医药大学附属医院,四川成都610000 [2]成都中医药大学,四川成都610000 [3]中山市中医院,广东中山528400

出　　处：《中医药信息》2023年第12期14-17,22,共5页Information on Traditional Chinese Medicine

基　　金：四川省中医院/成都中医药大学附属医院科技发展基金项目(21ZYTS01/y2021077)。

摘　　要：目的:通过观察文氏马齿苋汤对急性湿疹模型小鼠血清IgE、IL-2和IL-5水平的影响,为其治疗急性湿疹提供实验依据。方法:36只Balb/c雄性小鼠随机分为空白组、模型组、龙胆泻肝丸组和文氏马齿苋汤高、中、低剂量组,采用2,4-二硝基氯苯(DNCB)丙酮-橄榄油液激发小鼠急性湿疹,然后给予各组小鼠相应药物治疗7 d。疗程结束后采用酶联免疫吸附测定(ELISA)法检测各组IgE、IL-2和IL-5水平。结果:与空白组比较,文氏马齿苋汤各剂量组急性湿疹小鼠皮损均有所好转,表现在仅肉眼可见少量红斑、痂壳、鳞屑,其中文氏马齿苋汤高、中剂量组最为明显,且能不同程度降低血清IgE,IL-2和IL-5水平(P<0.05,P<0.01)。与龙胆泻肝丸组比较,文氏马齿苋汤中剂量组IgE表达更低(P<0.05),文氏马齿苋汤高剂量组IL-2水平明显降低(P<0.05),文氏马齿苋汤中、低剂量组IL-5表达水平显著降低(P<0.01,P<0.05)。结论:文氏马齿苋汤能显著降低急性湿疹模型小鼠血清IgE、IL-2、IL-5水平,同时减轻皮损红斑、水肿、渗出等症状。Objective:To provide an experimental basis for the treatment of acute eczema by observing the effects of Wenshi purslane decoction(WSPD)on the serum IgE,IL-2 and IL-5 levels in mice model with acute eczema.Methods:In this study,36 BALB/c male mice were selected and randomly divided into blank group,model group,gentian liver-draining pill group,high dose,medium dose,and low dose groups of WSPD.We used acetone-olive oil solution of 2,4-dinitrobenzone(DNCB)to induce acute eczema in mice,and then all the mice were treated for 7 days.After the experiment,the levels of IgE,IL-2 and IL-5 in each group were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the blank group,the skin lesions of acute eczema mice in all groups of WSPD were relieved,as only a small reduction of erythema,crust and scales could be observed.These changes are the most apparent in the high and middle dose groups of WSPD.Furthermore,WSPD could reduce serum IgE,IL-2 and IL-5 to varying degrees(P<0.05,P<0.01).Compared with gentian liver-draining pill group,the medium dose group of WSPD had lower IgE expression(P<0.05),the high dose group of WSPD had apparently lower IL-2 levels(P<0.05),and the medium and low dose groups of WSPD had significantly lower IL-5 expression levels(P<0.01,P<0.05).Conclusion:WSPD can significantly reduce the levels of serum IgE,IL-2 and IL-5 in mice models of acute eczema,and also alleviate the symptoms of erythema,edema and exudation of skin lesions.

关 键 词：急性湿疹 文氏马齿苋汤 IGE IL-2 IL-5 

分 类 号：R285.5[医药卫生—中药学]