复合酶辅助超声法提取鸡蛋花总黄酮工艺优化  

Extraction Technology Optimization of Total Flavonoids from Frangipani by Compound Enzyme Assisted Ultrasonography

作　　者：陆显进 王绍丽 朱鼎慧 温悦 LU Xian-jin;WANG Shao-li;ZHU Ding-hui;WEN Yue(College of Medical,Yunnan University of Business Management Medical,Kunming 650106,China)

机构地区：[1]云南经济管理学院医学院,昆明650106

出　　处：《中国食物与营养》2023年第12期11-19,共9页Food and Nutrition in China

基　　金：云南省教育厅科学研究基金项目“鸡蛋花总黄酮提取、纯化及护肤作用研究”(项目编号:2023J1496)。

摘　　要：目的:用响应面法优化复合酶辅助超声提取鸡蛋花总黄酮工艺,探求鸡蛋花总黄酮提取最佳工艺。方法:以鸡蛋花总黄酮得率为指标,对不同温度、不同料液比、不同乙醇浓度、不同pH等单因素进行考察,根据单因素试验结果,运用响应面法对提取工艺参数进行优化。结果:不同温度、不同料液比、不同乙醇浓度、不同pH等对鸡蛋花总黄酮得率有显著影响,得到鸡蛋花总黄酮得率最佳提取工艺条件是温度43℃、乙醇体积分数30%、料液比1∶60、pH=4.8、酶量3%、超声时间30 min,该条件下鸡蛋花的总黄酮平均得率为2.86%。结论:采用响应面法可较好地优化鸡蛋花总黄酮的提取工艺,该模型稳定可靠,具有一定的可行性,可为其开发和利用提供理论依据以及工艺参数。【Objective】Response Surface Method was used to optimize the extraction process of total flavonoids from frangipani by ultrasonic-assisted compound enzyme to explore the optimal extraction technology of total flavonoids from frangipani.【Method】The extrac⁃tion parameters were optimized by response surface method according to the experimental results of single factor such as different temperature,different solid-liquid ratio,different ethanol concentration,and different pH.【Result】Different temperature,different ratio of solid to liquid,different concentration of ethanol,and different pH had significant effects on the yield of total flavone.The optimal extraction conditions were temperature 43℃,ethanol volume fraction 30%,solid-liquid ratio 1∶60,pH 4.8,enzyme content 3%,and ultrasonic time 30 min.Under these conditions,the average yield of total flavonoids was 2.86%.【Conclusion】Response Surface Method can be used to optimize the extraction process of total flavonoids from frangipani.The model is stable,reliable and feasible,which can provide theoretical basis and technological parameters for its development and utilization.

关 键 词：鸡蛋花 总黄酮 复合酶 超声波提取法 工艺优化 

分 类 号：R284.2[医药卫生—中药学]