A crowd-sourced genomic project to assess hybrid content in a rare avian vagrant(Azure Tit Cyanistes cyanus(Pallas,1770))  

作　　者：Martin Irestedt Filip Thorn Per G.P.Ericson Hein van Grouw Yaroslav A.Red'kin Alexander Hellquist Frank Johansson Johan A.A.Nylander 

机构地区：[1]Department of Bioinformatics and Genetics,Swedish Museum of Natural History,Stockholm,Sweden [2]Department of Zoology Stockholm University,Sweden [3]Bird Group,Department of Life Sciences,Natural History Museum,Tring,United Kingdom [4]Zoological Museum,Lomonosov Moscow State University,Moscow,Russia [5]Institute of Biological Problems of Cryolithozone SB RAS,Yakutsk,Russia [6]Department of Women's and Children's Health,Uppsala University,Sweden [7]Department of Ecology and Genetics,Uppsala University,Uppsala,Sweden

出　　处：《Avian Research》2023年第4期545-552,共8页鸟类学研究（英文版）

基　　金：This project was crowd sourced and mainly financed by interested birdwatchers.MI also acknowledge financial support from the Swedish research council(2019-03900);Riksmusei vanner.

摘　　要：The aim of this study was to correlate plumage variation with the amount of genomic hybrid content in hybrids between Azure Tits Cyanistes cyanus(Pallas,1770)and European Blue Tit Cyanistes caeruleus(Linnaeus,1758),by re-sequencing the genomes of museum specimens of non-hybrids and presumed hybrids with varying plumages.The project was funded by crowdsourcing and initiated when two presumed Azure Tits,observed by hundreds of Swedish birdwatchers,were rejected as hybrids based on minor plumage deviations assumed to indicate hybrid contents from the European Blue Tit.The results confirm that hybrids with intermediate plumages,so called Pleske’s Tits,are first generation hybrids(F1 hybrids).Individuals,whose plumages are similar to Azure Tits,but assessed as hybrids based on minor plumage deviations,are all backcrosses but vary in their degree of hybrid content.However,some individuals morphologically recognized as pure Azure Tits expressed similar degrees of hybrid content.The results indicate that:(1)hybrid content may be widespread in Azure Tits in the western part of its habitat distribution;(2)plumage deviation in backcrosses is not linearly correlated with the genetic degree of hybrid origin;and(3)all Azure Tits observed in Europe outside its natural distribution may have some degree of hybrid origin.We therefore suggest that it is very difficult to phenotypically single out hybrids beyond first generation backcrosses.We argue that decreased sequencing costs and improved analytical tools open the doors for museomic crowd-sourced projects that may not address outstanding biological questions but have a major interest for lay citizens such as birdwatchers.

关 键 词：Crowdsourcing Cyanistes cyanus HYBRIDIZATION Museomics 

分 类 号：Q958.1[生物学—动物学]