基于半胱胺保护的金纳米酶催化光度法测定痕量亚硝酸根  

Colorimetric Detection of Nitrite Ion Based on Peroxidase Mimic Activity of Cysteamine-protected Gold Nanoparticles

作　　者：姚秀琪 韦砻 覃庆婷 何深怡 吴海娇 凌绍明 Yao Xiuqi;Wei Long;Qin Qingting;He Shenyi;Wu Haijiao;Ling Shaoming(College of Chemistry and Environment Engineering,Baise University,Baise 533000,China)

机构地区：[1]百色学院化学与环境工程学院,广西百色533000

出　　处：《山东化工》2023年第21期168-171,175,共5页Shandong Chemical Industry

基　　金：2021年百色学院国家级大学生创新创业训练计划项目资助(202110609036)。

摘　　要：在硫酸介质中,亚硝酸根催化溴酸钾氧化过量的碘化钾生成碘三负离子(I_(3)^(-)),I_(3)^(-)能够刻蚀半胱胺保护的金纳米粒子(cyst-AuNPs),导致cyst-AuNPs过氧化物模拟酶的催化活性被抑制,建立了简单、灵敏测定亚硝酸根的比色传感方法。优化了cyst-AuNPs模拟酶催化反应条件。在最佳试验条件下,方法的线性范围为0.00833~0.142μg·mL^(-1),线性回归方程ΔA=0.0249+3.474ρ,相关系数R^(2)为0.9877,检出限为0.0058μg·mL^(-1)。将方法应用于水样中亚硝酸根测定,测定结果的RSD在2.1%~3.8%,回收率在93%~107%。In sulfuric acid medium,nitrite ion catalyzed the reaction of potassium bromate and excess potassium iodide to generate triiodide ion(I _(3)^(-)),which could etched cysteamine-protected gold nanoparticles(cyst-AuNPs),resulting in inhibition of the peroxidase mimic activity of cyst-AuNPs.Therefore,a simple and sensitive colorimetric method for the determination of nitrite ion was established.The conditions of the catalytic reaction with cyst-AuNPs as peroxidase mimic were investigated.Under the optimal conditions,the linear range of the method was 0.00833~0.142μg·mL^(-1) and the linear regression equation wasΔA=0.0249+3.474ρ,with a correlation coefficient of R ^(2)=0.9877 and a detection limit of 0.0058μg·mL^(-1),respectively.The method was applied to the determination of nitrite in water samples with RSD of 2.1%~3.8%and recoverie of 93%~107%.

关 键 词：亚硝酸根 比色方法 半胱胺保护的金纳米粒子 过氧化物模拟酶活性 

分 类 号：O657.3[理学—分析化学]