猪口蹄疫O型灭活疫苗临床免疫效果评估  

Evaluation of Clinical Immune Effect of the Swine Foot and Mouth Disease Type O Inactivated Vaccine from Three Manufacturers

作　　者：张国新 张志新 张晓晓 帕提古丽 玉努斯·阿不都 马春江 ZHANG Guoxin;ZHANG Zhixin;ZHANG Xiaoxiao;Patiguli;YUNUSI Abudu;MA Chunjiang(Hami Animal Disease Prevention and Control Center,Hami,Xinjiang 839000,China;Yizhou Animal Disease Prevention and Control Center,Hami,Xinjiang 839000,China)

机构地区：[1]哈密市动物疫病预防控制中心,新疆哈密839000 [2]伊州区动物疫病预防控制中心,新疆哈密839000

出　　处：《家畜生态学报》2023年第12期74-77,共4页Journal of Domestic Animal Ecology

基　　金：口蹄疫、高致病性禽流感等重大动物疫病免疫效果评估模型的研究与应用-哈密市科学研究与技术开发计划项目(hm2021kj11)。

摘　　要：为准确掌握不同厂家猪口蹄疫O型灭活疫苗(O/Mya98/XJ/2021株+O/GX/09-7株)在生产中的临床免疫效果,筛选优质、可靠的猪口蹄疫O型灭活疫苗,本研究通过筛选哈密市某猪场同一批母源抗体保护水平较低的生猪(45 d~50 d),选用3个不同厂家的猪口蹄疫O型灭活疫苗分别对A、B、C 3组生猪进行免疫,并采用口蹄疫液相阻断ELISA方法检测免疫前后抗体效价。结果发现,首免28 d后免疫抗体均已达到较高水平,二免28 d后较首免后免疫抗体滴度明显升高,且二免60 d后免疫抗体合格率依旧维持在较高水平,在育肥猪出栏时疫苗免疫保护屏障较为完善,防护效果依然良好。对不同厂家疫苗免疫抗体滴度独立样本进行K-W检验,结果显示首免28 d后、二免28 d后和60 d后,A、B、C各组之间差异显著(P<0.05),A组疫苗免疫抗体滴度的中位数明显高于B、C 2组(P<0.05),且集中程度明显高于B、C 2组(P<0.05)。以上结果表明,A厂家猪口蹄疫O型灭活疫苗免疫抗体效价、维持时间、集中程度等免疫效果指标优于B、C 2个厂家。因此,建议本地生猪养殖场宜选用A厂家口蹄疫O型灭活疫苗,生猪45 d~50 d时进行首免,且在间隔28 d后实行二免,以保证生猪在出栏前仍对猪口蹄疫O型野毒具有较高的保护作用。In order to accurately know the clinical immune effect of swine type O foot and mouth disease inactivated vaccine(O/Mya98/XJ/2021 strain+O/GX/09-7 strain)of three manufacturers in production,and screen high-quality and reliable swine FMD O-type inactivated vaccine,in this study,the same batch of pigs(45 d~50 d)with low protection level of maternal antibody in a large-scale local pig farm were screened.FMD type O inactivated vaccines of three different manufacturers were selected to immunize pigs in groups A,B and C respectively,and the antibody titers before and after immunization were detected by FMD liquid phase blocking ELISA.Results showed that the immune antibodies reached a high level 28 days after the first immunization,and the titer of the second immunization was significantly higher than that of the first immunization,and the qualified rate of the immune antibodies remained at a high level 60 days after the second immunization.The immune protective barrier of the vaccine was relatively perfect and the protective effect was still good when the finishing pigs were sold to the market.After 28 days of the first vaccination,28 days of the second vaccination and 60 days of the second vaccination,the independent samples of immune antibody titers of different manufacturers were tested by K-W test.There were significant differences among groups A,B and C(P<0.05),and the median of immune antibody titers of group A was significantly higher than that of group B and C(P<0.05).The concentration degree was significantly higher than that of B and C groups(P<0.05).The above results showed that the immune effect indexes such as immune antibody titer,retention time and concentration degree of inactivated FMDV O vaccine in pigs produced by manufacturer A were better than those produced by manufacturers B and C.It is therefore suggested that the local pig farms use the inactivated FMDV O vaccine produced by manufacturer A,and immunize the pigs at 45 d to 50 d,and conduct second immunization after 28 d interval,so as to guara

关 键 词：口蹄疫病毒 灭活疫苗 ELISA 抗体水平 免疫效果 

分 类 号：S858.28[农业科学—临床兽医学]