纳米银胁迫下土壤微生物磷脂脂肪酸的响应特征  

Response Characteristics of Soil Microbial Phospholipid Fatty Acids Under Silver Nanoparticles Stress

作　　者：宋宇迪 樊昊心 姚槐应 SONG Yudi;FAN Haoxin;YAO Huaiying(School of Environmental Ecology and Biological engineering,Wuhan Institute of Technology,Wuhan 430205,China)

机构地区：[1]武汉工程大学环境生态与生物工程学院,湖北武汉430205

出　　处：《武汉工程大学学报》2023年第6期655-662,共8页Journal of Wuhan Institute of Technology

基　　金：武汉工程大学研究生教育创新基金(CX2021451)。

摘　　要：通过检测不同纳米银粒子(AgNPs)质量分数下(0、10、100、1 000 mg·kg^(-1))土壤中微生物标志物磷脂脂肪酸(PLFAs)含量的变化,探讨了AgNPs对农田土壤微生物群落结构的影响。结果表明:随AgNPs质量分数增加,土壤微生物PLFA总含量和微生物PLFA多样性指数均显著降低;革兰氏阳性菌标志脂肪酸a15:0和i16:0、真菌标志脂肪酸18:2ω6c,以及革兰氏阴性菌标志脂肪酸16:1ω7c和18:1ω7c的丰度在不同质量分数AgNPs处理组间表现出显著差异,该结果体现了不同微生物类群对AgNPs的差异性响应特征。AgNPs显著影响了土壤微生物PLFAs含量和群落结构特征,因此需要对AgNPs在农田土壤中的生态风险进行系统评估和科学监管。The influence of silver nanoparticles(AgNPs)on the microbial community structure in agricultural soil was investigated by assessing changes in the content of microbial biomarkers,namely phospholipid fatty acids(PLFAs),under different AgNPs mass fractions(0,10,100,1000 mg·kg^(-1)).The results reveal that both the total content of microbial PLFAs and the microbial PLFA diversity index significantly decrease with an increase in AgNPs mass fraction.The abundances of gram-positive bacterial marker fatty acids a15:0 and i16:0,fungal marker fatty acid 18:2ω6c,as well as gram-negative bacterial marker fatty acids 16:1ω7c and 18:1ω7c,exhibit significant variations among different AgNPs treatment groups,indicating distinctive responses of different microbial groups to AgNPs.AgNPs significantly impact the content and community structure of soil microbial PLFAs,highlighting the need for a systematic assessment and scientific regulation of the ecological risks of AgNPs in agricultural soil.

关 键 词：纳米银 土壤微生物 磷脂脂肪酸 胁迫 响应 

分 类 号：Q89[生物学]