微生物菌群的甲烷氧化效能及吸附煤体特性  

Methane oxidizing capacity and coal adsorbing characteristics of microbial flora

作　　者：周银波 张瑞林[1] 魏建平[2] 田坤云[1] 宫伟东 赵双 石涵锋 ZHOU Yinbo;ZHANG Ruilin;WEI Jianping;TIAN Kunyun;GONG Weidong;ZHAO Shuang;SHI Hanfeng(School of Resources and Safety Engineering,Henan University of Engineering,Zhengzhou,Henan 451191,China;School of Safety Science and Engineering,Henan Polytechnic University,Jiaozuo,Henan 454000,China;School of Energy Science and Engineering,Henan Polytechnic University,Jiaozuo,Henan 454000,China)

机构地区：[1]河南工程学院资源与安全工程学院 [2]河南理工大学安全科学与工程学院 [3]河南理工大学能源科学与工程学院

出　　处：《天然气工业》2023年第12期152-160,共9页Natural Gas Industry

基　　金：国家自然科学基金项目“煤的趋化作用及其改造煤吸附甲烷特性实验研究”(编号:52174168);河南省高校科技创新团队计划和创新人才支持计划项目“煤矿瓦斯治理与煤层气开发”(编号:22IRTSTHN009);“煤矿甲烷绿色减排”(编号:24HASTIT019);河南省科协青年人才托举工程项目“甲烷氧化菌在煤矿甲烷绿色减排和治理工作中的应用基础研究”(编号:2023HYTP014);河南省高校重点科研项目“二氧化碳驱替煤层瓦斯技术的气体储运特征研究”(编号:23A620004)。

摘　　要：甲烷氧化菌以消耗甲烷为能量来源,在环境甲烷减排方面具有广泛的应用价值,但在煤矿井下的应用研究仍处于起步阶段。为促进微生物技术在煤矿中的应用,提高煤层甲烷的治理效果,从自然界环境中筛选驯化出微生物菌群,借助实验培养装置监测了生长过程中菌液的微环境变化和气体消耗特征,并采用接触角测试装置和理论分析,考察了纯水、培养基和菌液在不同煤样表面的接触角动态变化,最后结合傅里叶红外光谱测试(FTIR)分析了取自山西沁城煤矿的无烟煤(QC)和取自河南新安煤矿的焦煤(XA)中矿物质对润湿角的影响规律。研究结果表明:①所筛选的微生物菌群具有较强的甲烷氧化特性,两组实验结果表明15天内甲烷降解率可达71.19%和84.63%,氧气降低率可达100%;②菌液中微生物生长密度OD600值增大30倍,在第6~8天内生长最为旺盛,菌液pH值由6.32上升至7.25,ORP则由210 mV下降至93 mV;③较之于纯水和培养基,菌液在煤体表面具有较强的润湿能力,在两种煤样表面的平衡接触角最小,XA煤样中含亲水性矿物较多,3种溶液的平衡接触角均小于QC煤样。结论认为,甲烷氧化菌群在煤矿井下具有较高的适应能力和应用价值,说明微生物技术在煤矿甲烷防治方面具有一定的优势,对煤矿安全生产具有重要意义。Methane-oxidizing bacteria gets energy by consuming methane,so it has wide application value in environmental methane emission reduction,but its application in coal mines underground is still in the stage of initial research.In order to promote the application of microbial technologies in coal mines and improve the treatment effect of coalbed methane,this paper selects microbial flora from the natural environment and acclimates them.Then,the microenvironmental change and gas consumption characteristics of the bacterial solution during the growth process are monitored with the experimental culture device,and the dynamic change of contact angle of pure water,culture medium and bacterial solution on the surface of different coal samples is investigated by means of contact angle measuring device and theoretical analysis.Finally,the influences of minerals in QC and XA coal samples,the former anthracite coal from Jincheng coal mine in Shanxi province and the latter coking coal from Xin'an Coal Mine in Henan Province,on wetting angle are analyzed by virtue of Fourier Transform Infrared Spectroscopy(FTIR).And the following research results are obtained.First,the selected microbial flora has strong methane oxidizing capacity.The results of two groups of experiment indicate that the methane degradation rate can reach 71.19% and 84.63% within 15 days,and the oxygen reduction rate can reach 100%.Second,the OD600 value of microbial growth density in the bacterial solution increases by 30 times,and the growth is the most vigorous from the 6th day to the 8th day.The pH value of bacterial solution increases from 6.32 to 7.25,and the ORP decreases from 210 mV to 93 mV.Third,compared with pure water and culture medium,bacterial solution has stronger wettability on the surface of coal and the smallest equilibrium contact angle on the surface of two kinds of coal samples.XA coal samples contain many hydrophilic minerals,and their equilibrium contact angles in three kinds of solution are smaller than that of QC coal samples.In conclu

关 键 词：甲烷减排 甲烷氧化菌 润湿角 甲烷氧化 消耗特征 甲烷降解率 生长特性 

分 类 号：TD712[矿业工程—矿井通风与安全]