活性氧在胚胎和生殖细胞中的研究进展及其检测方法  

Research progress and detection methods of reactive oxygen species in embryo and germ cells

作　　者：徐沛欣 陈红 王惠增 刘秉春[2] 袁建龙[1] XU Peixin;CHEN Hong;WANG Huizeng;LIU Bingchun;YUAN Jianlong(Department of Laboratory,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010000,China;Department of Achievement Transformation/Engineering Laboratory of Tumor Cell Gene Detection Application and Research,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010000,China)

机构地区：[1]内蒙古医科大学附属医院检验科,呼和浩特010000 [2]内蒙古医科大学附属医院成果转化科/肿瘤细胞基因检测应用与研究工程实验室,呼和浩特010000

出　　处：《生命的化学》2023年第10期1599-1608,共10页Chemistry of Life

基　　金：国家自然科学基金项目(31960154);“草原英才”工程青年创新创业人才项目(Q2022085);内蒙古自治区自然科学基金项目(2023QN03047);内蒙古自治区教育厅高等学校科学研究项目(NJZZ23017);内蒙古医科大学面上项目(YKD2022MS033);中央引导地方科技发展资金项目(2023ZY0004)。

摘　　要：近年来,我国人口增长速度显著减缓,现代人生育质量问题日益凸显。新生人口的日趋减少将对社会的可持续发展带来巨大挑战,与此同时我国的不孕症患病率同样居高不下。其中,引起生殖系统异常改变的一个重要因素是活性氧(reactive oxygen species,ROS)增高,引发机体氧化应激,从而造成生殖系统的损伤。ROS异常增高会导致胚胎发育停滞、精子形态异常、卵母细胞凋亡等,最终影响生殖健康。通过检测可以明确ROS的含量是否异常,从而实现早预防早干预,更加有助于保护生殖健康。本文拟通过对ROS生成异常对生殖细胞的不利影响、检测和清除异常ROS的技术手段进行总结与分析,为保护人类的生殖健康提供相关理论依据。In recent years,Chinaʼs population growth rate has slowed down significantly,and the problem of modern fertility quality has become increasingly prominent.The decrease of the new population will bring great challenges to the sustainable development of society,at the same time,the infertility prevalence rate in our country is also high.One of the important factors that cause abnormal changes in reproductive system is the increase of reactive oxygen species(ROS),which leads to oxidative stress in the body,resulting in damage to the reproductive system.Abnormal increase of ROS will lead to embryo development arrest,abnormal sperm morphology,oocyte apoptosis,etc.,which ultimately affects reproductive health.Through detection,it can be clear whether the ROS content is abnormal,so as to achieve early prevention and intervention,which is more helpful to protect reproductive health.This paper intends to summarize and analyze the adverse effects of abnormal ROS production on germ cells and the technical means for detecting and removing abnormal ROS,aiming to provide relevant theoretical basis for protecting human reproductive health.

关 键 词：活性氧 生殖健康 胚胎 精子 卵母细胞 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]