干旱胁迫对百脉根形态和生理指标的影响  

Effects of drought stress on morphological and physiological indexes of Lotus japonicus

作　　者：李亚娇 马培杰[1] 罗文举 舒健虹[1] 陈莹 龙忠富 王小利[1] LI Yajiao;MA Peijie;LUO Wenju;SHU Jianhong;CHEN Ying;LONG Zhongfu;WANG Xiaoli(Institute of Prataculture Reserch,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;College of Animal Sciences Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州省农业科学院草业研究所,贵阳550006 [2]贵州大学动物科学学院,贵阳550025

出　　处：《黑龙江畜牧兽医》2023年第24期85-93,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：贵州省重点基金项目(黔科合基础[2019]1449号);贵州省草业研究所科技创新课题(草业所科技创新课题[2018]03号);贵州省科研机构创新能力建设专项(黔科合服企[2022]004);贵州省高层次创新型人才培养项目(黔科合平台人才[2018]5634-2);国家牧草产业技术项目(CARS-34)。

摘　　要：为了研究干旱胁迫对百脉根形态和生理指标的影响,了解贵州地区百脉根的抗旱性能,试验设置5个处理,分别为P1(6%聚乙二醇)、P2(12%聚乙二醇)、P3(18%聚乙二醇)、P4(24%聚乙二醇)处理及空白对照(CK,以纯化水配制的霍格兰营养液)处理,每个处理15瓶,对苗期的百脉根进行干旱胁迫,测定胁迫30 d后百脉根的形态和生理指标。采用主成分分析和模糊隶属函数对不同聚乙二醇浓度处理下的百脉根进行综合评价。结果表明:随着干旱胁迫的增加,百脉根的株高、根长、根际直径、茎粗、地上鲜重、地下鲜重、单株鲜重大体上呈先降低后升高再降低的趋势,其中P2处理的根长、根际直径最长,茎粗最粗,各处理的株高、根体积差异不显著(P>0.05),CK处理的株高最高,P3处理的根体积最大;CK处理的地上鲜重最大,P2处理地下鲜重最大且显著高于CK、P1、P3及P4处理(P<0.05);随着干旱胁迫的增加,主根根系活力呈先升高后波动降低的趋势,须根根系活力呈先降低后增加再降低的趋势,叶部叶绿素含量呈先波动升高后降低的趋势,茎部叶绿素含量呈不断升高的趋势,其中P1处理主根根系活力最高,P2处理须根根系活力最高;叶部P3处理叶绿素含量最高,显著高于CK、P1及P2处理(P<0.05);茎部P4处理叶绿素含量最高,显著高于其他处理(P<0.05);随着干旱胁迫的增加,叶部和茎部的蛋白质含量、丙二醛(malondialdehyde,MDA)浓度、脯氨酸(proline,PRO)质量浓度、超氧化物歧化酶(superoxide dismutase,SOD)活性、过氧化氢酶(catalase,CAT)活性大体上呈波动上升趋势;叶部过氧化物酶(peroxidase,POD)活性呈先升高后降低的趋势,而茎部呈先降低后升高的趋势;乙烯(1-aminocyclopropane-1-carboxylic acid,ACC)含量呈波动下降趋势,脱落酸(abscisic acid,ABA)含量呈逐渐升高趋势,水杨酸(salicylic acid,SA)、3-吲哚乙酸(3-indoleacetic acid,IAA)含量呈先升高后降低的趋�In order to study the effects of drought stress on the morphology and physiological indicators of Lotus japonicus and understand its drought resistance performance in Guizhou region,five treatments were set up,namely P1(6%polyethylene glycol-6000),P2(12%polyethylene glycol-6000),P3(18%polyethylene glycol-6000),P4(24%polyethylene glycol-6000)treatment and blank control(CK,Hoagland nutrient solution prepared with purified water)treatment.Each treatment had 15 bottles,and lotus japonicus at the seedling stage was subjected to drought stress。The morphological and physiological indicators of Lotus japonicus were determined after 30 days of stress.The principal component analysis and fuzzy membership function were used to comprehensively evaluate the Lotus japonicus under different polyethylene glycol-6000 concentrations.The results showed that with the increase of drought stress,the plant height,root length,rhizosphere diameter,stem diameter,aboveground fresh weight,underground fresh weight,and single plant fresh weight of Lotus japonicus generally decreased first and then increased and then decreased.Among them,the root length,rhizosphere diameter,and stem diameter in the P2 treatment were the largest,and the differences in plant height and root volume among different treatments were not significant(P>0.05).The plant height in the CK treatment was the highest,while the root volume in the P3 treatment was the largest.The aboveground fresh weight was the highest in the CK treatment,and the underground fresh weight in the P2 treatment was the highest and significantly higher than that in the CK,P1,P3,and P4 treatments(P<0.05).With the increase of drougth stress,the main root activity showed a trend of first increasing and then fluctuating decreasing,while the fibrous root activity showed a trend of first decreasing,then increasing and then decreasing.The leaf chlorophyll content showed a trend of fluctuating increasing and then decreasing,while the stem chlorophyll content showed a continuous upward trend.Among them,t

关 键 词：百脉根 干旱胁迫 形态 生理指标 主成分分析 模糊隶属函数 综合评价值 

分 类 号：S541.6[农业科学—作物学]