超高效液相色谱-串联质谱法同时测定动物类中药材中6种伏马毒素  

Simultaneous determination of six fumonisins in animal traditional Chinese medicines by ultra performance liquid chromatography-tandem mass spectrometry

作　　者：胡珀[1] 何晓希 金华[1] 赵祥杰[2] 卜媛媛[1] HU Po;HE Xiaoxi;JIN Hua;ZHAO Xiangjie;BU Yuanyuan(Huai'an Institute for Food and Drug Control,Huai'an 223001,China;Huaiyin Institute of Technology,Huai'an 223001,China)

机构地区：[1]淮安市食品药品检验所,江苏淮安223001 [2]淮阴工学院,江苏淮安223001

出　　处：《化学分析计量》2023年第12期7-11,16,共6页Chemical Analysis And Meterage

基　　金：江苏省市场监督管理局科技计划项目(KJ2022048)。

摘　　要：建立超高效液相色谱-串联质谱法同时测定动物类中药材中6种伏马毒素。样品经20 mL 80%乙腈水溶液提取,离心后上清液用QuEChERS EMR-Lipid净化包净化,净化液采用超高效液相色谱-串联质谱法在多反应监测模式下测定6种伏马毒素含量,色谱峰面积外标法定量。6种伏马毒素在质量浓度为2~40 ng/mL范围内线性关系良好,相关系数均大于0.999,检出限为0.4~1.4μg/kg。在16、40、80μg/kg 3个加标浓度水平下,平均回收率为77.3%~101.6%,相对标准偏差为1.7%~8.4%(n=6)。该方法简单快速,定性定量准确,可用于动物类中药材中伏马毒素的检测。A method was established for the simultaneous determination of fumonisins in traditional Chinese animal medicines by ultra performance liquid chromatography-tandemmass spectrometry(UPLC-MS/MS).The samples were extracted with 20 mL 80%acetonitrile-water and purified by QuEChERS EMR-Lipid purification package after centrifugation.UPLC-MS/MS was employed for the quantification and confirmation of 6 fumonisins with the external standard method of chromatographic peak area.The linear relationships of 6 fumonisins was good in the range of 2-40 ng/mL,the correlation coefficients were all more than 0.999,the limit of detection were 0.4-1.4μg/kg.The average recoveries were 77.3%-101.6%with the relative standard deviations of 1.7%-8.4%(n=6)at three addition level of 16,40 and 80μg/kg.This method is simple and fast,accurate in qualitative and quantitative analysis,which can be used to detect fumonisins in traditional Chinese animal medicines.

关 键 词：超高效液相色谱-串联质谱法 动物类中药材 伏马毒素 

分 类 号：O657[理学—分析化学]