高效液相色谱法同时测定化妆品中7种禁用植物原料标志性成分  

Determination of 7 signature ingredients from prohibited raw material by HPLC

作　　者：宋佳 赖焕华 姜丽君 林叶婧 陈华龙 SONG Jia;LAI Huanhua;JIANG Lijun;LIN Yejing;CHEN Hualong(Shaoguan Institute for Food and Drug Control,Shaoguan 512028,China)

机构地区：[1]韶关市食品药品检验所,广东韶关512028

出　　处：《化学分析计量》2023年第12期17-22,共6页Chemical Analysis And Meterage

基　　金：广东省药品监督管理局化妆品禁用植(动)物组分检验检测技术重点项目(2020ZDB14);广东省药品监督管理局基金项目(2021TDB39);韶关市科技局基金项目(21081044531868)。

摘　　要：建立高效液相色谱法同时测定化妆品的巴豆苷、苦杏仁苷、花椒毒素、狼毒乙素、马兜铃酸、欧前胡素、土木香内酯7种化合物。采用资生堂CAPCELL PAK MG C18柱(250 mm×4.6 mm,5μm),以乙腈-0.05%磷酸为流动相梯度洗脱,流量为1 mL/min,进样体积为10μL,柱温为30℃,采用二极管阵列检测器,在210 nm波长处测定。7种化合物在所测浓度范围内有良好的线性关系,相关系数均大于0.999,平均回收率为90.0%~105.0%,测定结果的相对标准偏差为0.3%~2.8%(n=6),方法检出限为0.02~0.17μg/g。该方法可以高效、准确的检测化妆品中7种禁用植物原料的标志性成分。A high-performance liquid chromatography method was developed for rapidly detecting seven compounds including Crotonoside,Amygdalin,xanthotoxin.2,4-dihydroxy-6-methoxy-3 methylacetophenone,aristolochic acid,imperatorin and alantolactone.The analysis was carried out by Capcell PAK MGⅡC18 column(250 mm×4.6 mm,5μm),acetonitrile-water(containing 0.05%phosphoric acid)used as mobile phases for gradient elution,the flow rate was 1.0 mL/min.The injection volume was 10μL,the column temperature was 30℃,and the diode array detector was used,while the wavelength of 210 nm was selected for detection.The method had good linearity of 7 components in corresponding liner range with the correlation coefficients more than 0.999.The recoveries at low and high spiking levels were 90.0%-105.0%with the relative standard deviations ranged from 0.3%to 2.8%.The method detection limits were within the range of 0.02-0.17μg/g.This method was efficient,accurate for the determination of 7 signature ingredients from prohibited raw material.

关 键 词：化妆品 原料 高效液相色谱法 光敏性 

分 类 号：O657.7[理学—分析化学]