高效液相色谱法同时测定僵蚕中4种核苷类成分  

Simultaneous determination of four nucleosides in Bombyx mori L.by liquid chromatagraphy

作　　者：崔业波[1] 马晓静[1] 金鑫[1] 马彧[1] 王丹彧 CUI Yebo;MA Xiaojing;JIN Xin;MA Yu;WANG Danyu(Siping City Institute for Food and Drug Control,Siping 136000,China)

机构地区：[1]四平市食品药品检验所,吉林四平136000

出　　处：《化学分析计量》2023年第12期28-31,共4页Chemical Analysis And Meterage

基　　金：吉林省中药饮片炮制规范课题(JLPZGF-2020-062)。

摘　　要：建立高效液相色谱法测定僵蚕中尿苷、次黄嘌呤、腺嘌呤和腺苷的分析方法。样品用水提取后,用Polaris 5 C8-A色谱柱(250 mm×4.6 mm,5μm)分离,以甲醇-水为流动相梯度洗脱,流量为1.0 mL/min,柱温为30℃,采用紫外检测器检测,检测波长为260 nm,以色谱峰面积外标法定量。尿苷、次黄嘌呤、腺嘌呤和腺苷质量分别在0.041~0.305μg、0.047~0.350μg、0.040~0.298μg、0.041~0.310μg范围内与色谱峰面积线性关系良好,相关系数均大于0.9996,测定结果的相对标准偏差为1.2%~2.1%(n=6),样品加标平均回收率为94.6%~97.9%。该方法有助于僵蚕药材及其饮片的质量标准提高。A method for the determination of uridine,hypoxanthine,adenine and adenosine in Bombyx mori L.by high performance liquid chromatography was established.The samples were extracted with water and separated on a Polaris 5 C8-A column(250 mm×4.6 mm,5μm)with methanol-water as mobile phase by gradient elution at a flow rate of 1.0 mL/min.The column temperature was 30℃and the detection wavelength was 260 nm.The chromatographic peak area external standard method was used for quantification.The mass of uridine,hypoxanthine,adenine and adenosine had a good linear relationship with the chromatographic peak area in the range of 0.041-0.305μg,0.047-0.350μg,0.040-0.298μg,and 0.041-0.310μg,respectively.The correlation coefficients were more than 0.9996,and the relative standard deviations of the determination results were 1.2%-2.1%(n=6).The average recoveries were 94.6%-97.9%,respectively.This method improves the quality standards of silkworm medicinal materials and their decoction pieces.

关 键 词：高效液相色谱法 紫外检测器 僵蚕 尿苷 次黄嘌呤 腺嘌呤 腺苷 

分 类 号：O657.7[理学—分析化学]