二极管阵列检测器高效液相色谱法同时测定小柴胡片中10种指标成分  

Simultaneous determination of 10 indicator components in Xiaochaihu Tablets by HPLC-DAD

作　　者：崔俊凤 梁秀坤 崔帅 夏方亮 CUI Junfeng;LIANG Xiukun;CUI Shuai;XIA Fangliang(Binzhou Testing Center,Binzhou Key Laboratory of Chemical Drug R&D and Quality Control,Binzhou 256600,China)

机构地区：[1]滨州市检验检测中心,滨州市化学药物研发与质量控制重点实验室,山东滨州256600

出　　处：《化学分析计量》2023年第12期54-60,共7页Chemical Analysis And Meterage

摘　　要：建立二极管阵列检测器高效液相色谱法同时测定小柴胡片中柴胡皂苷a、柴胡皂苷d、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、党参炔苷、甘草苷、甘草酸和6-姜辣素的含量。该药物粉末在30℃水温用5%浓氨试液的甲醇溶液超声提取液和70%乙醇溶液加热回流提取液的等体积混合液作为样品溶液,分析采用Venusil XBP C_(18)(L)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,在波长210、237、280 nm下进行检测,体积流量为1.0 mL/min,柱温为25℃。10种指标成分在一定浓度范围内线性关系良好,相关系数均不小于0.9997,平均加标回收率为91.40%~97.82%,相对标准偏差为0.79%~2.88%(n=6)。该方法可用于小柴胡片的质量控制。A high performance liquid chromatography-diode array detector(HPLC-DAD)method was established for the simultaneous determination of bupleurum a,bupleurum d,baicalin,scutellarin,baicalein,wogonin,codonopsis alkynoside,glycyrrhizin,glycyrrhizic acid and 6-gingerols in Xiaochaihu Tablets.The drug powder was analyzed using a Venusil XBP C_(18)(L)chromatographic column(250 mm×4.6 mm,5μm),using an equal volume mixture of methanol solution ultrasonic extraction solution with 5%concentrated ammonia test solution and 70%ethanol solution heated reflux extraction solution at a water temperature of 30℃.Gradient elution was performed using acetonitrile 0.1%phosphoric acid solution as the mobile phase,and detection was performed at wavelengths 210,237,and 280 nm.The volume flow rate was 1.0 mL/min,and the column temperature was 25℃.Ten results showed good linear relationships within their own ranges with the corelation coefiicients not less than 0.9997,whose average recoveries were 91.40%-97.82%with the relative standard deviations of 0.79%-2.88%(n=6).This method can be used for quality control of Xiaochaihu Tablets.

关 键 词：小柴胡片 指标成分 高效液相色谱法 二极管阵列检测器 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]