聚岩藻多糖对小鼠巨噬细胞分泌炎症因子的影响及其机制  

Effect of polysaccharides from porphyra on inflammatory factors secreted by macrophages in mice and its mechanism

作　　者：周雪冰 林千千 李艳春[1] ZHOU Xuebing;LIN Qianqian;LI Yanchun(Department of Pediatric Respiratory,First Hospital,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院小儿呼吸科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2023年第6期1452-1456,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科技发展计划项目(20190201036JC);吉林省财政厅医疗卫生人才专项项目(JLSWSRCZX2021)。

摘　　要：目的:探讨聚岩藻多糖对脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7分泌炎性因子的影响,并阐明其免疫调节作用。方法:取对数生长期RAW264.7细胞,分为对照组、LPS组和LPS+聚岩藻多糖组,采用CCK-8法检测各组细胞存活率,酶联免疫吸附试验(ELISA)法检测各组细胞培养上清中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)、巨噬细胞炎性蛋白(MIP)-1α和MIP-1β水平。结果:与对照组比较,LPS组和LPS+聚岩藻多糖组细胞存活率明显升高(P<0.01),细胞培养上清中IL-6、TNF-α、MCP-1、MIP-1α和MIP-1β水平均明显升高(P<0.01)。与LPS组比较,LPS+聚岩藻多糖组细胞存活率明显降低(P<0.01),细胞培养上清中IL-6、TNF-α、MCP-1、MIP-1α和MIP-1β水平明显降低(P<0.01)。结论:聚岩藻多糖可抑制小鼠巨噬细胞对炎症因子IL-6、TNF-α、MCP-1、MIP-1α和MIP-1β分泌的影响,具有潜在的免疫调节作用。Objective:To discuss the effect of polysaccharides from porphyra on the inflammatory factors secreted by macrophage RAW264.7 cells in the mice induced by lipopolysaccharide(LPS),and to clarify their immunomodulatory effect.Methods:The RAW264.7 cells at logarithmic growth phase were divided into control group,LPS group,and LPS+polysaccharide from porphyra group.The viabilities of the cells in various groups were detected by CCK-8 method;the levels of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),monocyte chemotactic protein-1(MCP-1),macrophage inflammatory protein(MIP)-1α,and MIP-1βin the cell culture supernatants in various groups were detected by enzyme-linked immunosorbent assay(ELISA)method.Results:Compared with control group,the survival rate of the cells and the levels of IL-6,TNF-α,MCP-1,MIP-1α,and MIP-1βin the culture supernatant in LPS group and LPS+polysaccharide from porphyra group were significantly increased(P<0.01).Compared with LPS group,the survival rate of the cells and the levels of IL-6,TNF-α,MCP-1,MIP-1α,and MIP-1βin the culture supernatant in LPS+polysaccharide from porphyra group were significantly decreased(P<0.01).Conclusion:polysaccharides from porphyra can inhibit the impact of the mouse macrophages on the secretion of inflammatory factors IL-6,TNF-α,MCP-1,MIP-1α,and MIP-1β,and have the potential immunomodulatory effect.

关 键 词：聚岩藻多糖 免疫调节 巨噬细胞 炎症因子 脂多糖 

分 类 号：R282.77[医药卫生—中药学]