子痫前期患者胎盘组织中抑微管装配蛋白1表达对滋养层细胞的影响及其机制  

Effect of expression of microtubule inhibitory assembly protein 1in placenta tissue of pre-eclampsia patients on trophoblast cellsand its mechanism

作　　者：欧曼颖 胡春霞[1] 李跃萍[1,2] OU Manying;HU Chunxia;LI Yueping(Department of Obstetrics,First Affiliated Hospital,Hainan Medical College,Haikou 570100,China;Department of Histology and Embryology,Hainan Medical College,Haikou 570100,China)

机构地区：[1]海南医学院第一附属医院产科,海南海口570100 [2]海南医学院组织学与胚胎学教研室,海南海口570100

出　　处：《吉林大学学报（医学版）》2023年第6期1519-1527,共9页Journal of Jilin University：Medicine Edition

基　　金：海南省卫健委卫生健康行业科研项目(20A200141)。

摘　　要：目的:探讨子痫前期(PE)患者胎盘组织中抑微管装配蛋白1(STMN1)表达对滋养层细胞侵袭和迁移功能的影响,并阐明其相关机制。方法:选取17名健康妊娠孕产妇(对照组)和15例PE患者(PE组)的胎盘组织。将人滋养层HTR-8细胞分为siRNA-STMN1组(转染siRNA-STMN1)、siRNA-NC组(转染阴性对照序列siRNA-NC)、pcDNA3.1-STMN1组(转染pcDNA3.1-STMN1)和pcDNA3.1-NC组(转染阴性对照序列pcDNA3.1-NC)。采用免疫组织化学(IHC)染色观察2组研究对象胎盘组织中STMN1和上皮-间质转化(EMT)相关蛋白表达强度,CCK-8法检测各组HTR-8细胞增殖率,细胞划痕实验检测各组HTR-8细胞迁移能力,Transwell小室实验检测各组HTR-8细胞侵袭能力,实时荧光定量PCR(RT-qPCR)法检测2组研究对象和各组HTR-8细胞中STMN1 mRNA表达水平,Western blotting检测2组研究对象和各组HTR-8细胞中STMN1、E-钙黏蛋白(E-cadherin)和N-钙黏蛋白(N-cadherin)蛋白表达水平。结果:与对照组比较,PE组患者收缩压、舒张压和尿蛋白水平均明显升高(P<0.01),新生儿体质量增加(P<0.05)。IHC染色观察,与对照组比较,PE组患者胎盘绒毛组织中STMN1和E-cadherin蛋白表达强度增加,N-cadherin蛋白表达强度降低。CCK-8法检测,与siRNA-NC组比较,siRNA-STMN1组HTR-8细胞增殖率降低(P<0.05);与pcDNA3.1-NC组比较,pcDNA3.1-STMN1组HTR-8细胞增殖率升高(P<0.05)。细胞划痕实验检测,与siRNA-NC组比较,siRNA-STMN1组HTR-8细胞迁移率降低(P<0.05);与pcDNA3.1-NC组比较,pcDNA3.1-STMN1组HTR-8细胞迁移率升高(P<0.05)。Transwell小室实验检测,与siRNA-NC组比较,siRNA-STMN1组侵袭细胞数明显减少(P<0.01);与pcDNA3.1-NC组比较,pcDNA3.1-STMN1组侵袭细胞数明显增加(P<0.01)。RT-qPCR法检测,与对照组比较,PE组患者胎盘组织中STMN1 mRNA表达水平降低(P<0.05);与siRNA-NC组比较,siRNA-STMN1组HTR-8细胞中STMN1 mRNA表达水平明显降低(P<0.01);与pcDNA3.1-NC组比较,pcDNA3.1-STMN1组HTR-8细胞中STMN1 mRNA表�Objective:To discuss the effect of the expression of stathmin 1(STMN1)in placenta tissue of the pre-eclampsia(PE)patients on the invasion and migration functions of the trophoblast cells,and to clarify the related mechanism.Methods:The placenta tissues of 17 healthy pregnant women(control group)and 15 PE patients(PE group)were selected.The human trophoblast HTR-8 cells were divided into siRNA-STMN1 group(transfected with siRNA-STMN1),siRNA-NC group(transfected with negative control sequence siRNA-NC),pcDNA3.1-STMN1 group(transfected with pcDNA3.1-STMN1),and pcDNA3.1-NC group(transfected with negative control sequence pcDNA3.1-NC).The expression intensities of STMN1 and epithelial-mesenchymal transition(EMT)-related proteins in placenta tissue of the patients in two groups were observed by immunohistochemistry(IHC)staining;the proliferation rates of the HTR-8 cells in various groups were detected by CCK-8 method;the migration abilities of the HTR-8 cells in various groups were detected by cell scratch experiment;the invasion abilities of the HTR-8 cells in various groups were detected by Transwell chamber experiment;the expression levels of STMN1 mRNA in placenta tissue of the patients in two groups and HTR-8 cells were detected by real-time fluorescence quantitative PCR(RT-qPCR)method;the expression levels of STMN1,E-cadherin,and N-cadherin proteins in placenta tissue of the patients in two groups and HTR-8 cells were detected by Western blotting method.Results:Compared with control group,the systolic blood pressure,diastolic blood pressure,and the level of urine protein of the patients in PE group were significantly increased(P<0.01),and the body weight of the newborn was increased(P<0.05).The IHC staining results showed that compared with control group,the expression intensities of STMN1 and E-cadherin proteins in placenta tissue of the patients in PE group were increased,and the expression intensity of N-cadherin protein was decreased.The CCK-8 results showed that compared with siRNA-NC group,the proliferation

关 键 词：子痫前期 抑微管装配蛋白1 滋养层细胞 细胞增殖 细胞侵袭 上皮-间质转化 

分 类 号：R714.25[医药卫生—妇产科学]