小青龙汤变方在COPD大鼠气道炎症反应中的作用机制探讨  

Exploring the mechanism of the effect of Xiaoqinglong Decoction Modified Prescription on airway inflammatory response in COPD rats

作　　者：汪新龙[1] 梁波流 张鹏程 梁志科 刘朝晖 WANG Xin-long;LIANG Bo-liu;ZHANG Peng-cheng(Nansha Hospital of Guangzhou First People's Hospital,Guangzhou 511457,China)

机构地区：[1]广州市第一人民医院南沙医院,广州511457 [2]华南理工大学附属第二医院,广州510180

出　　处：《中国处方药》2023年第12期54-57,共4页Journal of China Prescription Drug

基　　金：广东省中医药局科研项目(20221296)。

摘　　要：目的研究慢性阻塞性肺疾病(COPD)大鼠模型中白细胞介素-6、肿瘤坏死因子-ɑ、干扰素-γ在大鼠血清和肺泡灌洗液中含量变化,探索我国传统经典方剂小青龙汤变方在COPD气道炎症治疗中的作用机制。方法成年雄性SD大鼠60只,分为对照组20只,模型组20只,干预组20只,采用反复熏香烟+气管内注入脂多糖法建立COPD大鼠动物模型,其中干预组予灌胃小青龙汤变方。酶联免疫吸附法(ELISA法)检测大鼠血清、肺泡灌洗液中IL-6、TNF-ɑ和IFN-γ的含量。结果在模型组中,血清和肺泡灌洗液中TNF-α和IL-6较干预组均显著升高,IFN-γ表达下降。结论COPD大鼠模型中,小青龙汤变方可能影响气道炎症因子水平,从而一定程度改善气道炎症。Objective To study interleukin-6,tumor necrosis factor-ɑand Interferon-γin rat model of chronic obstructive pulmonary disease(COPD),in order to explore the mechanism of Xiaoqinglong Decoction Modified Prescription in the treatment of COPD airway inflammation,the content changes in rat serum and alveolar lavage fluid were observed.Methods Sixty adult male SD rats were divided into the control group(20 rats),the model group(20 rats)and the intervention group(20 rats).The animal model of COPD rats was established by repeated cigarette smoking and intratracheal injection of lipopolysaccharide.The intervention group was fed with Xiaoqinglong Decoction Modified Prescription.Detection of IL-6,TNF-ɑand IFN-γin rat serum and alveolar lavage fluid by enzyme-linked immunosorbent assay content was measured by ELISA.Results In the model group,TNF-αin serum and alveolar lavage fluid and IL-6 significantly higher than the intervention group,IFN-γexpression decreased.Conclusion In the COPD rat model,the Xiaoqinglong Decoction Modified Prescription may affect the level of airway inflammatory factors,thus improve airway inflammation to a certain extent.

关 键 词：慢性阻塞性肺疾病 气道炎症 小青龙汤变方 白细胞介素-6 肿瘤坏死因子-α 干扰素-Γ 

分 类 号：R285.5[医药卫生—中药学]