白细胞介素13调控人鼻黏膜上皮基底细胞增殖  

Interleukin-13 regulates the proliferation of human nasal mucosal epithelial basal cells

作　　者：赵超然 王明[1] 王成硕[1] 张罗[1,2] ZHAO Chaoran;WANG Ming;WANG Chengshuo;ZHANG Luo(Department of Otolaryngology Head and Neck Surgery,Key Laboratory of Otolaryngology Head and Neck Surgery(Capital Medical University),Beijing Institute of Otolaryngology,Engineering Research Center of Ministry of Education,Beijing Key Laboratory of Nasal Diseases,Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases,Chinese Academy of Medical Sciences,Capital Medical University,Beijing,100730,China;Department of Allergy,Beijing Tongren Hospital,Capital Medical University,Beijing,100730,China)

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京市耳鼻咽喉科研究所,教育部工程中心,鼻病研究北京市重点实验室,中国医学科学院慢性鼻病创新单元,北京100730 [2]首都医科大学附属北京同仁医院变态反应科,北京100730

出　　处：《中国耳鼻咽喉头颈外科》2023年第11期723-726,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家重点研发计划(2022YFC2504100);教育部长江学者创新团队(IRT13082);中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-022);国家自然科学基金(82171109、81870698);北京市医院管理中心“登峰”计划(DFL20190202)。

摘　　要：目的 探究慢性鼻窦炎伴鼻息肉(CRSwNP)鼻黏膜上皮基底细胞增殖情况,以及白细胞介素13(IL-13)对基底细胞增殖的影响。方法 收集健康对照及CRSwNP患者鼻黏膜组织,并对组织进行切片、免疫荧光染色及real-time PCR检测。分离培养原代人鼻黏膜上皮细胞,经IL-13处理后利用real-time PCR结合免疫荧光法检测基底细胞增殖及相关基因的表达。结果 与对照组相比,CRSwNP患者上皮中表达肿瘤蛋白p63(TP63)的基底细胞数量显著增加;TP63、细胞增殖标志物Ki67(MKI67)、炎症因子IL-13在转录表达水平显著升高;IL-13的表达与TP63、MKI67的表达呈显著正相关。原代鼻黏膜上皮细胞经IL-13刺激,显著促进TP63+基底细胞的增殖,以及TP63、MKI67基因的表达。结论 CRSwNP鼻黏膜上皮基底细胞增殖显著增加可能与炎症因子IL-13的调控有关。OBJECTIVETo investigate the cell proliferation in the nasal mucosa of patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and the effect of interleukin-13(IL-13)on basal cell proliferation.METHODS Nasal mucosa tissues from healthy controls and patients with CRSwNP were collected and subjected to sectioning,immunofluorescence staining and real-time PCR.Primary human nasal mucosal epithelial cells were isolated and cultured,and after IL-13 treatment,basal cell proliferation and related gene expression were detected by real-time PCR and immunofluorescence staining.RESULTSCompared with the control group,the number of basal cells expressing tumor protein p63(TP63)was significantly increased in the epithelium of CRSwNP patients;TP63,cell proliferation marker Ki67(MKI67),and the inflammatory factor IL-13 were significantly elevated in the level of transcriptional expression;and IL-13 expression was significantly positively correlated with TP63,MKI67 expression showed a significant positive correlation.Primary nasal mucosal epithelial cells stimulated by IL-13 significantly promoted the proliferation of TP63+basal cells and the expression of TP63 and MKI67 genes.CONCLUSION Basal cell proliferation was significantly increased in CRSwNP nasal mucosal tssues,which may be regulated by the inflammatory factor IL-13.

关 键 词：鼻窦炎 鼻息肉 白细胞介素13 鼻粘膜 上皮 基底细胞增殖 

分 类 号：R765[医药卫生—耳鼻咽喉科]