生长环境土壤水分对垂盆草利湿退黄药效及黄酮类成分的影响研究  

Effect of soil moisture on efficacy to eliminate dampness and relieve jaundice and flavonoid content of Sedum sarmentosum

作　　者：晏梦恬 史红专[1] 郭巧生[1] 江汇瀛 朱雅婷 朱再标[1] YAN Meng-tian;SHI Hong-zhuan;GUO Qiao-sheng;JIANG Hui-ying;ZHU Ya-ting;ZHU Zai-biao(Institute of Chinese Medicinal Materials,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2023年第21期5750-5758,共9页China Journal of Chinese Materia Medica

基　　金：中央高校基本科研业务费项目(KYZ201734)。

摘　　要：药用植物生长环境对其药材品质形成具有重要作用,但目前鲜见药用植物生长环境-化学成分-相关活性作用紧密结合的联合分析研究,因此该文探讨不同土壤水分处理对垂盆草利湿退黄功效与黄酮类成分的影响及其相关性。比较35%~40%(T1)、55%~60%(T2)、75%~80%(T3)、95%~100%(T4)田间持水量条件下生长的垂盆草其水煎液中黄酮类成分含量,以及对湿热黄疸小鼠肝功能指标、肝脏炎症和氧化损伤情况的影响,并对药理指标和黄酮类成分进行相关性分析。结果表明,垂盆草水煎液中总黄酮和总酚含量均以T1处理最高,T3处理次之,槲皮素、山柰酚及异鼠李素含量则分别以T2、T1、T3处理为优。不同水分处理垂盆草以T3组降低小鼠血清谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、总胆红素(TBIL)、直接胆红素(DBIL)、总胆汁酸(TBA)及肝脏肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平效果最好,T2处理次之,且对谷草转氨酶(AST)降低效果最佳,T4处理效果最差。相关性分析表明,小鼠AST、ALT、AKP水平与槲皮素及3种黄酮成分总量呈显著负相关;丙二醛(MDA)与总黄酮及山柰酚含量呈显著负相关;TNF-α与异鼠李素含量呈显著负相关。综上,土壤含水量为田间持水量的75%~80%条件下生长的垂盆草利湿退黄功效最佳。该研究为以药理活性实验为指导,优化药用植物栽培模式提供了思路。The growth environment of medicinal plants plays an important role in the formation of their medicinal quality.However,there is a lack of combined analysis studying the close relationship between the growth environment,chemical components,and related biological activities of medicinal plants.Therefore,this study investigated the effect of different soil moisture treatments on the efficacy to eliminate dampness and relieve jaundice and the flavonoid content of Sedum sarmentosum,and explored their correlation.The flavonoid content in the decoction of S.sarmentosum growing under field conditions with soil moisture levels of 35%-40%(T1),55%-60%(T2),75%-80%(T3),and 95%-100%(T4) was compared.The effects of these treatments on liver function parameters,liver inflammation,and oxidative damage in mice with dampness-heat jaundice were evaluated,and the correlation between pharmacological indicators and flavonoid content was analyzed.The results showed that the total flavonoid and total phenolic acid content in the decoction of S.sarmentosum were highest in the T1 treatment,followed by the T3 treatment.The content of quercetin,kaempferol,and isorhamnetin was highest in the T2,T1,and T3 treatments,respectively.Among the different moisture treatments,the T3 group of S.sarmentosum effectively reduced the levels of serum ALT,AKP,TBIL,DBIL,TBA,as well as hepatic TNF-α and IL-6 in mice with jaundice,followed by T2 treatment,especially in reducing AST level.The T4 treatment had the poorest effect.Correlation analysis showed a significant negative correlation between AST,ALT,AKP levels in mice and the total content of quercetin and the three flavonoids.MDA showed a significant negative correlation with the total flavonoid content and kaempferol.TNF-α exhibited a significant negative correlation with the content of isorhamnetin.In conclusion,S.sarmentosum growing under field conditions with a soil moisture level of 75%-80% exhibited the best efficacy to eliminate dampness and relieve jaundice.This study provides insights for optim

关 键 词：垂盆草 黄酮 利湿退黄 环境水分 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]