酒萸肉中2个新的苯丙素类成分  

Two new phenylpropanoids from wine-processed Corni Fructus

作　　者：王静[1,2] 池军 王佩 曹斌 张玲霞[1,2] 王智民[2,3] 代丽萍[1,2] WANG Jing;CHI Jun;WANG Pei;CAO Bin;ZHANG Ling-xia;WANG Zhi-min;DAI Li-ping(Henan University of Chinese Medicine,Zhengzhou 450046,China;Engineering Technology Research Center for Comprehensive Development and Utilization of Authentic Medicinal Materials in Henan Province,Henan University of Chinese Medicine,Zhengzhou 450046,China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学河南省豫产道地药材综合开发利用工程技术研究中心,河南郑州450046 [3]中国中医科学院中药研究所中药质量控制技术国家工程实验室,北京100700

出　　处：《中国中药杂志》2023年第21期5809-5816,共8页China Journal of Chinese Materia Medica

基　　金：河南省重大科技专项(221100310400);2023年度河南省高校科技创新团队项目(23IRTSTHN028);河南省中医药产业守正创新发展规划研究项目(TCM2021013);河南省科技研发计划联合基金(优势学科培育类)重点项目(222301420023)。

摘　　要：综合运用硅胶、ODS、Sephadex LH-20柱色谱和反相制备型HPLC等多种分离技术,结合HR-ESI-MS、UV、IR、NMR、ECD等波谱学方法,从酒萸肉水提液中共分离鉴定出6个化合物,分别为pinoresinoside B(1)、cornusgallicacid A(2)、(+)-isolariciresinol-9′-O-β-glucopyranoside(3)、(-)-isolariciresinol 3α-O-β-D-glucopyranoside(4)、(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(5)、开环异落叶松脂素-9′-O-β-D-葡萄糖苷(6)。其中化合物1和2为新化合物。生物活性评价结果表明,化合物2和6具有较强的DPPH自由基清除能力,其EC_(50)分别为(4.18±1.96)、(21.45±1.19)μmol·L^(-1);化合物1和2对H_(2)O_(2)诱导的大鼠肾细胞(NRK-52E)氧化损伤均具有保护作用,且呈剂量依赖性,其中化合物2在100μmol·L^(-1)浓度下的细胞存活率达96.09%±1.77%。Six compounds were isolated from aqueous extract of wine-processed Corni Fructus through silica gel,ODS column chromatography,Sephadex LH-20 gel column chromatography,reverse phase preparative HPLC and other chromatographic separation technologies.Their structures were identified with multiple spectroscopical methods including HR-ESI-MS,UV,IR,NMR and ECD and so on.Their structures were established as pinoresinoside B(1),cornusgallicacid A(2),(+)-isolariciresinol-9′-O-β-glucopyranoside(3),(-)-isolariciresinol 3α-O-β-D-glucopyranoside(4),(7R,8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside(5),and(-)-seco isolariciresinol-9′-O-β-D-glucopyranoside(6).Among them,compounds 1 and 2 were two new compounds.The biological activity evaluation results showed that compounds 2 and 6 had strong DPPH free radical scavenging ability,with EC_(50) values of(4.18±1.96) and(21.45±1.19) μmol·L~(-1),respectively.Compounds 1 and 2 had protective effects on H_2O_2-induced oxidative damage in NRK-52E cells in a dose-dependent manner,and the cell survival rate of compound 2 at 100 μmol·L~(-1) was 96.09%±1.77%.

关 键 词：酒萸肉 化学成分 苯丙素类 生物活性 

分 类 号：R284.1[医药卫生—中药学]