山竹果壳中1个新的呫吨酮类成分及其细胞毒活性  

A new xanthone from hulls of Garcinia mangostana and its cytotoxic activity

作　　者：赵锋宁 牛琦 肖碟 许浩楠 王浩鑫 毕荣璐 何红平 江志勇 ZHAO Feng-ning;NIU Qi;XIAO Die;XU Hao-nan;WANG Hao-xin;BI Rong-lu;HE Hong-ping;JIANG Zhi-yong(Chuxiong Yunzhi Pharmaceutical Co.,Ltd.,Chuxiong 675005,China;Key Laboratory of Modern Research on Ethnic Medicine in Yunnan Provincial Universities,Ethnomedicine School,Yunnan University of Chinese Medicine,Kunming 650500,China)

机构地区：[1]楚雄云植药业有限公司,云南楚雄675005 [2]云南中医药大学民族医药学院云南省高校民族药现代研究重点实验室,云南昆明650500

出　　处：《中国中药杂志》2023年第21期5817-5821,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81860620);云南省中医药联合专项重点项目(202101AZ070001-164);云南省教育厅高原特色民族药用植物研究科技创新团队项目。

摘　　要：采用硅胶柱色谱、Sephadex LH-20柱色谱以及半制备高效液相色谱等手段对山竹果壳的80%乙醇提取物的乙酸乙酯部分进行了化学成分研究,从中分离鉴定了8个化合物。通过现代波谱学分析,将该8个化合物的结构分别鉴定为16-en mangostenone E (1)、α-山竹黄酮(α-mangostin,2)、1,7-dihydroxy-2-(3-methylbut-2-enyl)-3-methoxyxanthone(3)、cratoxyxanthone (4)、2,6-dimethoxy-para-benzoquinone (5)、苔色酸甲酯(methyl orselinate,6)、ficusol (7)、4-(4-carboxy-2-methoxyphenoxy)-3,5-dimethoxybenzoic acid (8)。其中化合物1为新的呫吨酮类成分,化合物4为已知的呫吨酮二聚体,化合物5为苯醌。除化合物2、3外,其余化合物均为首次从该植物中分离得到。采用MTT法测定了所有化合物的细胞毒活性,结果显示化合物1、2、4对HeLa细胞有一定的抑制作用,其IC_(50)分别为24.3、35.5、17.1μmol·L^(-1)。化合物4对K562细胞有一定的抑制作用(IC_(50)为39.8μmol·L^(-1))。Eight compounds were isolated from ethyl acetate fraction of 80% ethanol extract of the hulls of Garcinia mangostana by silica gel,Sephadex LH-20 column chromatography,as well as prep-HPLC methods.By HR-ESI-MS,MS,1D and 2D NMR spectral analyses,the structures of the eight compounds were identified as 16-en mangostenone E(1),α-mangostin(2),1,7-dihydroxy-2-(3-methy-lbut-2-enyl)-3-methoxyxanthone(3),cratoxyxanthone(4),2,6-dimethoxy-para-benzoquinone(5),methyl orselinate(6),ficusol(7),and 4-(4-carboxy-2-methoxyphenoxy)-3,5-dimethoxybenzoic acid(8).Compound 1 was a new xanthone,and compound 4 was a xanthone dimer,compound 5 was a naphthoquinone.All compounds were isolated from this plant for the first time except compounds 2 and 3.Cytotoxic bioassay suggested that compounds 1,2 and 4 possessed moderate cytotoxicity,suppressing HeLa cell line with IC_(50) va-lues of 24.3,35.5 and 17.1 μmol·L~(-1),respectively.Compound 4 also could suppress K562 cells with an IC_(50) value of 39.8 μmol·L~(-1).

关 键 词：藤黄科 山竹 呫吨酮 细胞毒 

分 类 号：R284.1[医药卫生—中药学]